Print Email Facebook Twitter Salt dependence of the radius of gyration and flexibility of single-stranded DNA in solution probed by small-angle x-ray scattering Title Salt dependence of the radius of gyration and flexibility of single-stranded DNA in solution probed by small-angle x-ray scattering Author Sim, A.Y.L. Lipfert, J. Herschlag, D. Doniach, S. Faculty Electrical Engineering, Mathematics and Computer Science Department Bionanoscience Date 2012-08-01 Abstract Short single-stranded nucleic acids are ubiquitous in biological processes; understanding their physical properties provides insights to nucleic acid folding and dynamics. We used small-angle x-ray scattering to study 8–100 residue homopolymeric single-stranded DNAs in solution, without external forces or labeling probes. Poly-T's structural ensemble changes with increasing ionic strength in a manner consistent with a polyelectrolyte persistence length theory that accounts for molecular flexibility. For any number of residues, poly-A is consistently more elongated than poly-T, likely due to the tendency of A residues to form stronger base-stacking interactions than T residues. To reference this document use: http://resolver.tudelft.nl/uuid:9dc2ae7e-9726-4951-aa9f-6e0367c6faa0 DOI https://doi.org/10.1103/PhysRevE.86.021901 Publisher American Physical Society ISSN 1539-3755 Source http://link.aps.org/doi/10.1103/PhysRevE.86.021901 Source Physical Review E, 86 (2), 2012 Part of collection Institutional Repository Document type journal article Rights © 2012 American Physical Society Files PDF Lipfert_2012.pdf 586.97 KB Close viewer /islandora/object/uuid:9dc2ae7e-9726-4951-aa9f-6e0367c6faa0/datastream/OBJ/view