Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager

Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager

Giraud, G.
Schulze, H.
Li, D.U.
Bachmann, T.T.
Crain, J.
Tyndall, D.
Richardson, J.
Walker, R.
Stoppa, D.
Charbon, E.
Henderson, R.
Arlt, J.

Electrical Engineering, Mathematics and Computer Science

Micro Electronics

2010-11-04

Fluorescence lifetime of dye molecules is a sensitive reporter on local microenvironment which is generally independent of fluorophores concentration and can be used as a means of discrimination between molecules with spectrally overlapping emission. It is therefore a potentially powerful multiplexed detection modality in biosensing but requires extremely low light level operation typical of biological analyte concentrations, long data acquisition periods and on-chip processing capability to realize these advantages. We report here fluorescence lifetime data obtained using a CMOS-SPAD imager in conjunction with DNA microarrays and TIRF excitation geometry. This enables acquisition of single photon arrival time histograms for a 320 pixel FLIM map within less than 26 seconds exposure time. From this, we resolve distinct lifetime signatures corresponding to dye-labelled HCV and quantum-dot-labelled HCMV nucleic acid targets at concentrations as low as 10 nM.

fluorescence microscopy
lifetime-based sensing
medical optics instrumentation
biological sensing and sensors
avalanche photodiodes (APDs)

http://resolver.tudelft.nl/uuid:9f53395d-db0c-4d1d-9871-e847c967094f

https://doi.org/10.1364/BOE.1.001302

Optical Society of America

2156-7085

http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-1-5-1302

Biomedical Optics Express, 1 (5), 2010

Institutional Repository

journal article

(c) 2010 Optical Society of America