Print Email Facebook Twitter Analysis of centrifugal homogenization and its applications for emulsification & mechanical cell lysis Title Analysis of centrifugal homogenization and its applications for emulsification & mechanical cell lysis Author Singh, Kaustub (Student TU Delft) Gupta, Ankur (Princeton University) Buchner, A.J.L.L. (TU Delft Fluid Mechanics) Ibis, F. (TU Delft Intensified Reaction and Separation Systems) Pronk, J.W. (TU Delft BN/Arjen Jakobi Lab) Tam, D.S.W. (TU Delft Fluid Mechanics) Eral, H.B. (TU Delft Intensified Reaction and Separation Systems; Universiteit Utrecht) Date 2019 Abstract We detail the analysis of centrifugal homogenization process by a hydrodynamic model and the model-guided design of a low-cost centrifugal homogenizer. During operation, centrifugal force pushes a multiphase solution to be homogenized through a thin nozzle, consequently homogenizing its contents. We demonstrate and assess the homogenization of coarse emulsions into relatively monodisperse emulsions, as well as the application of centrifugal homogenization in the mechanical lysis of mpkCCD mouse kidney cells. To gain insight into the homogenization mechanism, we investigate the dependence of emulsion droplet size on geometrical parameters, centrifugal acceleration, and dispersed phase viscosity. Our experimental results are in qualitative agreement with models predicting the droplet size. Furthermore, they indicate that high shear rates kept constant throughout operation produce more monodisperse droplets. We show this ideal homogenization condition can be realized through hydrodynamic model-guided design minimizing transient effects inherent to centrifugal homogenization. Moreover, we achieved power densities comparable to commercial homogenizers by model guided optimization of homogenizer design and experimental conditions. Centrifugal homogenization using the proposed homogenizer design thus offers a low-cost alternative to existing technologies as it is constructed from off-the-shelf parts (Falcon tubes, syringe, needles) and used with a centrifuge, readily available in standard laboratory environment. Subject Centrifugal emulsificationCentrifugationEmulsionsHomogenizerLysis To reference this document use: http://resolver.tudelft.nl/uuid:a53d14d5-3abc-4f99-9df0-d0f923eafeb7 DOI https://doi.org/10.1016/j.jcis.2019.03.036 Embargo date 2020-09-20 ISSN 0021-9797 Source Journal of Colloid and Interface Science, 547, 127-135 Bibliographical note Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2019 Kaustub Singh, Ankur Gupta, A.J.L.L. Buchner, F. Ibis, J.W. Pronk, D.S.W. Tam, H.B. Eral Files PDF 1_s2.0_S0021979719303261_main.pdf 1.86 MB Close viewer /islandora/object/uuid:a53d14d5-3abc-4f99-9df0-d0f923eafeb7/datastream/OBJ/view