Print Email Facebook Twitter Distortion of DNA Origami on Graphene Imaged with Advanced TEM Techniques Title Distortion of DNA Origami on Graphene Imaged with Advanced TEM Techniques Author Kabiri, Y. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Ananth, A.N. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) van der Torre, J. (TU Delft BN/Technici en Analisten; Kavli institute of nanoscience Delft) Katan, A.J. (TU Delft QN/Afdelingsbureau; Kavli institute of nanoscience Delft) Hong, Jin Yong (Massachusetts Institute of Technology) Malladi, S.R.K. (TU Delft QN/Zandbergen Lab; Kavli institute of nanoscience Delft) Kong, Jing (Massachusetts Institute of Technology) Zandbergen, H.W. (TU Delft QN/Zandbergen Lab; Kavli institute of nanoscience Delft) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Date 2017-08-18 Abstract While graphene may appear to be the ultimate support membrane for transmission electron microscopy (TEM) imaging of DNA nanostructures, very little is known if it poses an advantage over conventional carbon supports in terms of resolution and contrast. Microscopic investigations are carried out on DNA origami nanoplates that are supported onto freestanding graphene, using advanced TEM techniques, including a new dark-field technique that is recently developed in our lab. TEM images of stained and unstained DNA origami are presented with high contrast on both graphene and amorphous carbon membranes. On graphene, the images of the origami plates show severe unwanted distortions, where the rectangular shape of the nanoplates is significantly distorted. From a number of comparative control experiments, it is demonstrated that neither staining agents, nor screening ions, nor the level of electron-beam irradiation cause this distortion. Instead, it is suggested that origami nanoplates are distorted due to hydrophobic interaction of the DNA bases with graphene upon adsorption of the DNA origami nanoplates. Subject DNA origamigraphenenanostructurestransmission electron microscopy To reference this document use: http://resolver.tudelft.nl/uuid:a95c0f70-e05f-4ff7-9587-444911b810cf DOI https://doi.org/10.1002/smll.201700876 Embargo date 2018-06-16 ISSN 1613-6810 Source Small (online), 13 (31), 1-8 Part of collection Institutional Repository Document type journal article Rights © 2017 Y. Kabiri, A.N. Ananth, J. van der Torre, A.J. Katan, Jin Yong Hong, S.R.K. Malladi, Jing Kong, H.W. Zandbergen, C. Dekker Files PDF smll.201700876_R1.pdf 1.59 MB Close viewer /islandora/object/uuid:a95c0f70-e05f-4ff7-9587-444911b810cf/datastream/OBJ/view