Print Email Facebook Twitter The emerging landscape of single-molecule protein sequencing technologies Title The emerging landscape of single-molecule protein sequencing technologies Author Bohländer, P.R. (TU Delft ChemE/Advanced Soft Matter) Filius, M. (TU Delft BN/Chirlmin Joo Lab; Kavli institute of nanoscience Delft) van Kooten, Xander F. (Technion) Pomorski, A.K. (TU Delft BN/Chirlmin Joo Lab; Kavli institute of nanoscience Delft) Schmid, S. (Wageningen University & Research) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Eelkema, R. (TU Delft ChemE/Advanced Soft Matter) Kim, S.H. (TU Delft BN/Chirlmin Joo Lab; Kavli institute of nanoscience Delft) Joo, C. (TU Delft BN/Chirlmin Joo Lab; Kavli institute of nanoscience Delft) Date 2021 Abstract Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics. To reference this document use: http://resolver.tudelft.nl/uuid:ac0665c1-ce46-443f-b861-ee52a86584bd DOI https://doi.org/10.1038/s41592-021-01143-1 Embargo date 2021-12-07 ISSN 1548-7091 Source Nature Methods (online), 18 (6), 604-617 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type review Rights © 2021 P.R. Bohländer, M. Filius, Xander F. van Kooten, A.K. Pomorski, S. Schmid, C. Dekker, R. Eelkema, S.H. Kim, C. Joo, More Authors Files PDF Manuscript_03_10_2021_201 ... ef_TU3.pdf 1.71 MB Close viewer /islandora/object/uuid:ac0665c1-ce46-443f-b861-ee52a86584bd/datastream/OBJ/view