Print Email Facebook Twitter Biocatalytically induced surface modification of the tobacco mosaic virus and the bacteriophage M13 Title Biocatalytically induced surface modification of the tobacco mosaic virus and the bacteriophage M13 Author Vignali, Valentina (Rijksuniversiteit Groningen) S. Miranda, Barbara (University of Campinas; Rijksuniversiteit Groningen) Lodoso-Torrecilla, Irene (Rijksuniversiteit Groningen) Van Nisselroy, Cathelijn A.J. (Rijksuniversiteit Groningen) Hoogenberg, Bas Jan (Rijksuniversiteit Groningen) Dantuma, Sybren (Rijksuniversiteit Groningen) Hollmann, F. (TU Delft BT/Biocatalysis) De Vries, Jan Willem (Rijksuniversiteit Groningen) Date 2019 Abstract Engineered viruses are finding an increasing number of applications in basic, translational research and materials science. Genetic and chemical engineering of capsids represents a key point for tailoring the properties of viral particles, but the synthetic efforts and limits accompanying these processes still hinder their usability. Here, a single-step highly selective biocatalytic functionalization approach is described, providing a general platform for virus-acrylate hybrid particles. The tobacco mosaic virus (TMV) and the bacteriophage M13 have been successfully modified via laccase induced free radical formation on the tyrosine residues through single electron oxidation as the initiating step and the free radicals subsequently react with acrylate-based monomers. This new approach can be extended to other biomolecular assemblies with surface exposed tyrosine residues, when the introduction of new functionalities is desired. To reference this document use: http://resolver.tudelft.nl/uuid:af667cd2-94cc-4f54-a560-f534d2c411ee DOI https://doi.org/10.1039/c8cc08042a Embargo date 2019-05-28 ISSN 1359-7345 Source Chemical Communications, 55 (1), 51-54 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2019 Valentina Vignali, Barbara S. Miranda, Irene Lodoso-Torrecilla, Cathelijn A.J. Van Nisselroy, Bas Jan Hoogenberg, Sybren Dantuma, F. Hollmann, Jan Willem De Vries Files PDF c8cc08042a.pdf 2.04 MB Close viewer /islandora/object/uuid:af667cd2-94cc-4f54-a560-f534d2c411ee/datastream/OBJ/view