Print Email Facebook Twitter Diffuse reflectance spectroscopy as a tool for real-time tissue assessment during colorectal cancer surgery Title Diffuse reflectance spectroscopy as a tool for real-time tissue assessment during colorectal cancer surgery Author Baltussen, Elisabeth J.M. (Netherlands Cancer Institute) Snaebjornsson, Petur (Netherlands Cancer Institute) De Koning, Susan G.Brouwer (Netherlands Cancer Institute) Sterenborg, Henricus J.C.M. (Netherlands Cancer Institute; Amsterdam UMC) Aalbers, Arend G.J. (Netherlands Cancer Institute) Kok, Niels (Netherlands Cancer Institute) Beets, Geerard L. (Netherlands Cancer Institute) Hendriks, B.H.W. (TU Delft Medical Instruments & Bio-Inspired Technology; Philips Research) Kuhlmann, Koert F.D. (Netherlands Cancer Institute) Ruers, Theo J.M. (Netherlands Cancer Institute; University of Twente) Date 2017 Abstract Colorectal surgery is the standard treatment for patients with colorectal cancer. To overcome two of the main challenges, the circumferential resection margin and postoperative complications, real-time tissue assessment could be of great benefit during surgery. In this ex vivo study, diffuse reflectance spectroscopy (DRS) was used to differentiate tumor tissue from healthy surrounding tissues in patients with colorectal neoplasia. DRS spectra were obtained from tumor tissue, healthy colon, or rectal wall and fat tissue, for every patient. Data were randomly divided into training (80%) and test (20%) sets. After spectral band selection, the spectra were classified using a quadratic classifier and a linear support vector machine. Of the 38 included patients, 36 had colorectal cancer and 2 had an adenoma. When the classifiers were applied to the test set, colorectal cancer could be discriminated from healthy tissue with an overall accuracy of 0.95 (±0.03). This study demonstrates the possibility to separate colorectal cancer from healthy surrounding tissue by applying DRS. High classification accuracies were obtained both in homogeneous and inhomogeneous tissues. This is a fundamental step toward the development of a tool for real-time in vivo tissue assessment during colorectal surgery. Subject colorectal cancerdiffuse reflectance spectroscopymachine learningmargin assessmentsupport vector machine To reference this document use: http://resolver.tudelft.nl/uuid:b23e1211-18b7-43c1-9b0c-c9316d22c768 DOI https://doi.org/10.1117/1.JBO.22.10.106014 ISSN 1083-3668 Source Journal of Biomedical Optics, 22 (10) Part of collection Institutional Repository Document type journal article Rights © 2017 Elisabeth J.M. Baltussen, Petur Snaebjornsson, Susan G.Brouwer De Koning, Henricus J.C.M. Sterenborg, Arend G.J. Aalbers, Niels Kok, Geerard L. Beets, B.H.W. Hendriks, Koert F.D. Kuhlmann, Theo J.M. Ruers Files PDF 106014.pdf 1.15 MB Close viewer /islandora/object/uuid:b23e1211-18b7-43c1-9b0c-c9316d22c768/datastream/OBJ/view