Hepatic glucose production is more sensitive to insulin-mediated inhibition than hepatic VLDL-triglyceride production
article
Insulin is an important inhibitor of both hepatic glucose output and hepatic VLDL-triglyceride (VLDL-TG) production. We investigated whether both processes are equally sensitive to insulin-mediated inhibition. To test this, we used euglycemic clamp studies with four increasing plasma concentrations of insulin in wild-type C57Bl/6 mice. By extrapolation, we estimated that half-maximal inhibition of hepatic glucose output and hepatic VLDL-TG production by insulin were obtained at plasma insulin levels of ∼3.6 and ∼6.8 ng/ml, respectively. In the same experiments, we measured that half-maximal decrease of plasma free fatty acid levels and half-maximal stimulation of peripheral glucose uptake were reached at plasma insulin levels of ∼3.0 and ∼6.0 ng/ml, respectively. We conclude that, compared with insulin sensitivity of hepatic glucose output, peripheral glucose uptake and hepatic VLDL-TG production are less sensitive to insulin. Copyright © 2006 the American Physiological Society. Chemicals / CAS: insulin, 9004-10-8; Blood Glucose; Fatty Acids, Nonesterified; Glucose, 50-99-7; Hypoglycemic Agents; Insulin, 11061-68-0; Lipoproteins, VLDL; Triglycerides
Topics
Biomedical ResearchEuglycemic hyperinsulinemic clampFatty acidVery-low-density lipoproteinInsulinAdipose tissueAnimal experimentAanimal modelControlled studyDrug effectDrug inhibitionFatty acid blood levelGluconeogenesisGlucose infusionGlucose transportHyperinsulinemiaInsulin blood levelInsulin resistanceInsulin sensitivityLipolysisLiver functionMouseNonhumanAnimalsBlood GlucoseDose-Response Relationship, DrugFatty Acids, NonesterifiedGlucoseGlucose Clamp TechniqueHypoglycemic AgentsInsulinKineticsLipoproteins, VLDLLiverMaleMiceMice, Inbred C57BLTriglycerides
TNO Identifier
239769
ISSN
01931849
Source
American Journal of Physiology - Endocrinology and Metabolism, 291(6)
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