Print Email Facebook Twitter Coherent Fourier scatterometry reveals nerve fiber crossings in the brain Title Coherent Fourier scatterometry reveals nerve fiber crossings in the brain Author Menzel, Miriam (TU Delft ImPhys/Menzel group) Pereira, S.F. (TU Delft ImPhys/Optics) Date 2020 Abstract Previous simulation studies by Menzel et al. [Phys. Rev. X 10, 021002 (2020)] have shown that scattering patterns of light transmitted through artificial nerve fiber constellations contain valuable information about the tissue substructure such as the individual fiber orientations in regions with crossing nerve fibers. Here, we present a method that measures these scattering patterns in monkey and human brain tissue using coherent Fourier scatterometry with normally incident light. By transmitting a non-focused laser beam (λ = 633 nm) through unstained histological brain sections, we measure the scattering patterns for small tissue regions (with diameters of 0.1-1 mm), and show that they are in accordance with the simulated scattering patterns. We reveal the individual fiber orientations for up to three crossing nerve fiber bundles, with crossing angles down to 25°. To reference this document use: http://resolver.tudelft.nl/uuid:b85ca8e9-67ea-4fa2-88db-7e7bf7e7e6fc DOI https://doi.org/10.1364/BOE.397604 ISSN 2156-7085 Source Biomedical Optics Express, 11 (8), 4735-4758 Part of collection Institutional Repository Document type journal article Rights © 2020 Miriam Menzel, S.F. Pereira Files PDF boe_11_8_4735.pdf 15.09 MB Close viewer /islandora/object/uuid:b85ca8e9-67ea-4fa2-88db-7e7bf7e7e6fc/datastream/OBJ/view