Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

Title

Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

 Author

Tosstorff, Andreas (DECHEMA Research Institute)
Kroner, Cora (DECHEMA Research Institute)
Opperman, Diederik J. (University of the Free State)
Hollmann, F. (TU Delft BT/Biocatalysis) ORCID 0000-0003-4821-756X
Holtmann, Dirk (DECHEMA Research Institute)

Date

2017

 Abstract

Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.


 Subject

C=C reduction
Design of experiments
Electroenzymatic synthesis
Mediated electron transfer
Old yellow enzyme

 To reference this document use:

http://resolver.tudelft.nl/uuid:bbe2acac-2a1c-42f8-b667-abda872538f2

 DOI

https://doi.org/10.1002/elsc.201600158

 Embargo date

2017-10-14

 ISSN

1618-0240

 Source

Engineering in Life Sciences, 17 (1), 71-76

 Part of collection

Institutional Repository

 Document type

journal article

 Rights

© 2017 Andreas Tosstorff, Cora Kroner, Diederik J. Opperman, F. Hollmann, Dirk Holtmann
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