Print Email Facebook Twitter Pupil mask diversity for image correction in microscopy Title Pupil mask diversity for image correction in microscopy Author Wilding, D. (TU Delft Team Raf Van de Plas) Pozzi, P. (TU Delft Team Raf Van de Plas) Soloviev, O.A. (TU Delft Team Raf Van de Plas; ITMO University; Flexible Optical B.V.) Vdovin, Gleb (TU Delft Team Raf Van de Plas; ITMO University; Flexible Optical B.V.) Verhaegen, M.H.G. (TU Delft Team Raf Van de Plas) Date 2018 Abstract Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned in several di erent orientations within the pupil, followed by computer processing of the diversity images. The method has proved to be fast, easy to implement, and cost-e ective in high-resolution imaging of casper fli:GFP zebrafish. To reference this document use: http://resolver.tudelft.nl/uuid:bc6cbe87-f72a-4071-b6c3-34ccd0943d4a DOI https://doi.org/10.1364/OE.26.014832 ISSN 1094-4087 Source Optics Express, 26 (12), 14832-14841 Part of collection Institutional Repository Document type journal article Rights © 2018 D. Wilding, P. Pozzi, O.A. Soloviev, Gleb Vdovin, M.H.G. Verhaegen Files PDF oe_26_12_14832.pdf 9.27 MB Close viewer /islandora/object/uuid:bc6cbe87-f72a-4071-b6c3-34ccd0943d4a/datastream/OBJ/view