Microchip electrophoresis for UVC-induced DNA damage assessment

Microchip electrophoresis for UVC-induced DNA damage assessment

Tierolf, A.D.H.

Lee, Y.K. (mentor)
French, P.J. (mentor)

Electrical Engineering, Mathematics and Computer Science

Microelectronics & Computer Engineering

2009-11-12

In this thesis a design is presented which main objectives are to improve the speed, sensitivity and resolution of the commonly used gel electrophoresis method. To this end a background study is performed to look at the differences and similarities between both gel and micro-chip electrophoresis. Based on these findings some calculations are done to see if the expected design can theoretically improve in the above mentioned fields. A design is created and later fabricated in order to obtain these objectives. For the design a glass substrate is used with integrated electrodes, combined with a PMT sensor system to process the signals from the chip via a computer program. The main techniques used for the fabrication are wet etching and wafer bonding. The testing results produce a clear signal, which shows improvements in sensitivity and resolution, as well as producing results in less than three minutes.

electrophoresis
micro-chip

http://resolver.tudelft.nl/uuid:becdcccc-4ef8-4eac-91df-d77571c629de

2009-11-12

Student theses

master thesis

(c) 2009 Tierolf, A.D.H.