Print Email Facebook Twitter Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization Title Interplay between the gold nanoparticle sub-cellular localization, size, and the photon energy for radiosensitization Author Lechtman, Eli (University of Toronto) Pignol, J.P.M. (TU Delft RST/Applied Radiation & Isotopes; Erasmus MC) Date 2017 Abstract There are large variations in the reported efficiency of gold nanoparticle (GNP) radiosensitization. We have previously reported on a predictive model, which accounts for the detailed Auger and photoelectron tracks to calculate the cell survival probability. After validating our model using PC-3 cells incubated with 2 mg/ml of 30 nm GNPs and irradiated with 100 kVp or 300 kVp beams, we evaluated the interplay between photon energy, GNP size (1.9 and 100 nm) and sub-cellular localization. Experiments were in excellent agreement with the model. In predictive modeling, using a 100 kVp source and 1.9 nm nanoparticles, GNP localization had a significant impact on cell survival. A sensitizer enhancement ratio of 1.34 was achieved when GNPs were localized outside the cells, increasing to 2.56 when GNPs were also distributed in the cytoplasm and nucleus. Using a 300 kVp source, which emits photons mainly above the gold K-edge, the dependence on GNP localization and size was barely detectable, since long ranged electrons dominate the energy deposition. In summary, achieving intracellular uptake with targeted-GNPs can significantly enhance radiosensitization for photon energies below the gold K-edge, where Auger electrons contribute significantly to the local energy deposition. For higher energies, this is much less important. To reference this document use: http://resolver.tudelft.nl/uuid:c55f0f31-cd78-47f5-906e-31d6351f10c9 DOI https://doi.org/10.1038/s41598-017-13736-y ISSN 2045-2322 Source Scientific Reports, 7 (1) Part of collection Institutional Repository Document type journal article Rights © 2017 Eli Lechtman, J.P.M. Pignol Files PDF s41598_017_13736_y.pdf 1.25 MB Close viewer /islandora/object/uuid:c55f0f31-cd78-47f5-906e-31d6351f10c9/datastream/OBJ/view