Print Email Facebook Twitter The Genetic Makeup and Expression of the Glycolytic and Fermentative Pathways Are Highly Conserved Within the Saccharomyces Genus Title The Genetic Makeup and Expression of the Glycolytic and Fermentative Pathways Are Highly Conserved Within the Saccharomyces Genus Author Boonekamp, F.J. (TU Delft BT/Industriele Microbiologie) Dashko, S. (TU Delft BT/Industriele Microbiologie) van den Broek, M.A. (TU Delft BT/Industriele Microbiologie) Gehrmann, T. (Westerdijk Fungal Biodiversity Institute) Daran, J.G. (TU Delft BT/Industriele Microbiologie) Daran-Lapujade, P.A.S. (TU Delft BT/Industriele Microbiologie) Date 2018 Abstract The ability of the yeast Saccharomyces cerevisiae to convert glucose, even in the presence of oxygen, via glycolysis and the fermentative pathway to ethanol has played an important role in its domestication. Despite the extensive knowledge on these pathways in S. cerevisiae, relatively little is known about their genetic makeup in other industrially relevant Saccharomyces yeast species. In this study we explore the diversity of the glycolytic and fermentative pathways within the Saccharomyces genus using S. cerevisiae, Saccharomyces kudriavzevii, and Saccharomyces eubayanus as paradigms. Sequencing data revealed a highly conserved genetic makeup of the glycolytic and fermentative pathways in the three species in terms of number of paralogous genes. Although promoter regions were less conserved between the three species as compared to coding sequences, binding sites for Rap1, Gcr1 and Abf1, main transcriptional regulators of glycolytic and fermentative genes, were highly conserved. Transcriptome profiling of these three strains grown in aerobic batch cultivation in chemically defined medium with glucose as carbon source, revealed a remarkably similar expression of the glycolytic and fermentative genes across species, and the conserved classification of genes into major and minor paralogs. Furthermore, transplantation of the promoters of major paralogs of S. kudriavzevii and S. eubayanus into S. cerevisiae demonstrated not only the transferability of these promoters, but also the similarity of their strength and response to various environmental stimuli. The relatively low homology of S. kudriavzevii and S. eubayanus promoters to their S. cerevisiae relatives makes them very attractive alternatives for strain construction in S. cerevisiae, thereby expanding the S. cerevisiae molecular toolbox. Subject glycolysispromoter characterizationSaccharomyces cerevisiaeSaccharomyces kudriavzeviiSaccharomyces eubayanustranscription factor binding sites To reference this document use: http://resolver.tudelft.nl/uuid:c85c963b-9dbe-403c-9a0e-1862695987ab DOI https://doi.org/10.3389/fgene.2018.00504 ISSN 1664-8021 Source Frontiers in Genetics, 9 Part of collection Institutional Repository Document type journal article Rights © 2018 F.J. Boonekamp, S. Dashko, M.A. van den Broek, T. Gehrmann, J.G. Daran, P.A.S. Daran-Lapujade Files PDF fgene_09_00504.pdf 5.45 MB Close viewer /islandora/object/uuid:c85c963b-9dbe-403c-9a0e-1862695987ab/datastream/OBJ/view