Print Email Facebook Twitter A Straightforward Approach for 3D Bacterial Printing Title A Straightforward Approach for 3D Bacterial Printing Author Lehner, B. (TU Delft BN/Stan Brouns Lab) Schmieden, D.T. (TU Delft BN/Marie-Eve Aubin-Tam Lab) Meyer, A.S. (TU Delft BN/Anne Meyer Lab) Date 2017-07-21 Abstract Sustainable and personally tailored materials production is an emerging challenge to society. Living organisms can produce and pattern an extraordinarily wide range of different molecules in a sustainable way. These natural systems offer an abundant source of inspiration for the development of new environmentally friendly materials production techniques. In this paper, we describe the first steps toward the 3-dimensional printing of bacterial cultures for materials production and patterning. This methodology combines the capability of bacteria to form new materials with the reproducibility and tailored approach of 3D printing systems. For this purpose, a commercial 3D printer was modified for bacterial systems, and new alginate-based bioink chemistry was developed. Printing temperature, printhead speed, and bioink extrusion rate were all adapted and customized to maximize bacterial health and spatial resolution of printed structures. Our combination of 3D printing technology with biological systems enables a sustainable approach for the production of numerous new materials. Subject 3D printingalginateEscherichia colisynthetic biology To reference this document use: http://resolver.tudelft.nl/uuid:c9511f73-4a86-46b6-9257-d9fd3e0dcf96 DOI https://doi.org/10.1021/acssynbio.6b00395 ISSN 2161-5063 Source ACS Synthetic Biology, 6 (7), 1124-1130 Part of collection Institutional Repository Document type journal article Rights © 2017 B. Lehner, D.T. Schmieden, A.S. Meyer Files PDF acssynbio.6b00395_1.pdf 4.28 MB Close viewer /islandora/object/uuid:c9511f73-4a86-46b6-9257-d9fd3e0dcf96/datastream/OBJ/view