Print Email Facebook Twitter DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex Title DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex Author Ketterer, Philip (Technische Universität München) Ananth, A.N. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Laman Trip, J.D.S. (TU Delft OLD BN/Hyun Youk Lab; Kavli institute of nanoscience Delft) Mishra, Ankur (University Medical Center Groningen) Bertosin, Eva (Technische Universität München) Ganji, M. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) van der Torre, J. (TU Delft BN/Technici en Analisten; Kavli institute of nanoscience Delft) Onck, Patrick (University Medical Center Groningen) Dietz, Hendrik (Technische Universität München) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Date 2018-03-02 Abstract The nuclear pore complex (NPC) is the gatekeeper for nuclear transport in eukaryotic cells. A key component of the NPC is the central shaft lined with intrinsically disordered proteins (IDPs) known as FG-Nups, which control the selective molecular traffic. Here, we present an approach to realize artificial NPC mimics that allows controlling the type and copy number of FG-Nups. We constructed 34 nm-wide 3D DNA origami rings and attached different numbers of NSP1, a model yeast FG-Nup, or NSP1-S, a hydrophilic mutant. Using (cryo) electron microscopy, we find that NSP1 forms denser cohesive networks inside the ring compared to NSP1-S. Consistent with this, the measured ionic conductance is lower for NSP1 than for NSP1-S. Molecular dynamics simulations reveal spatially varying protein densities and conductances in good agreement with the experiments. Our technique provides an experimental platform for deciphering the collective behavior of IDPs with full control of their type and position. To reference this document use: http://resolver.tudelft.nl/uuid:ca1f7c4b-974f-4147-a7d7-a21644a494ec DOI https://doi.org/10.1038/s41467-018-03313-w ISSN 2041-1723 Source Nature Communications, 9 (1) Part of collection Institutional Repository Document type journal article Rights © 2018 Philip Ketterer, A.N. Ananth, J.D.S. Laman Trip, Ankur Mishra, Eva Bertosin, M. Ganji, J. van der Torre, Patrick Onck, Hendrik Dietz, C. Dekker Files PDF s41467_018_03313_w.pdf 2.22 MB Close viewer /islandora/object/uuid:ca1f7c4b-974f-4147-a7d7-a21644a494ec/datastream/OBJ/view