Print Email Facebook Twitter Shack-Hartmann sensor based optical quality testing of whole slide imaging systems for digital pathology Title Shack-Hartmann sensor based optical quality testing of whole slide imaging systems for digital pathology Author Shakeri, S.M. Hulsken, B. Van Vliet, L.J. Stallinga, S. Faculty Applied Sciences Department ImPhys/Imaging Physics Date 2015-03-05 Abstract Whole Slide Imaging (WSI) systems are used in the emerging field of digital pathology for capturing high-resolution images of tissue slides at high throughput. We present a technique to measure the optical aberrations of WSI systems using a Shack-Hartmann wavefront sensor as a function of field position. The resulting full-field aberration maps for the lowest order astigmatism and coma are analyzed using nodal aberration theory. According to this theory two coefficients describe the astigmatism and coma inherent to the optical design and another six coefficients are needed to describe the cumulative effects of all possible misalignments on astigmatism and coma. The nodal aberration theory appears to fit well to the experimental data. We have measured and analyzed the full-field aberration maps for two different objective lens-tube lens assemblies and found that only the optical design related astigmatism coefficient differed substantially between the two cases, but in agreement with expectations. We have also studied full-field aberration maps for intentional decenter and tilt and found that these affect the misalignment coefficient for constant coma (decenter) and the misalignment coefficient for linear astigmatism (tilt), while keeping all other nodal aberration theory coefficients constant Subject digital pathologyWhole Slide ImagingShack-Hartmann wavefront sensoroptical transfer functionnodal aberration theoryastigmatismcoma To reference this document use: http://resolver.tudelft.nl/uuid:cb828b7e-7aa9-4fb5-ac67-363546999856 Publisher SPIE ISBN 9781628414059 Source https://doi.org/10.1117/12.2077085 Source Design and Quality for Biomedical Technologies VIII: Proceedings of SPIE- International Society for Optical Engineering; 9315 Part of collection Institutional Repository Document type conference paper Rights © 2015 SPIE Files PDF 323766.pdf 1.65 MB Close viewer /islandora/object/uuid:cb828b7e-7aa9-4fb5-ac67-363546999856/datastream/OBJ/view