Print Email Facebook Twitter Natronospira proteinivora gen. nov., sp. nov, an extremely salttolerant, alkaliphilic gammaproteobacterium from hypersaline soda lakes Title Natronospira proteinivora gen. nov., sp. nov, an extremely salttolerant, alkaliphilic gammaproteobacterium from hypersaline soda lakes Author Sorokin, Dimitry Y. (TU Delft BT/Environmental Biotechnology; Russian Academy of Sciences) Kublanov, Ilya V. (Russian Academy of Sciences; Immanuel Kant Baltic Federal University) Khijniak, Tatiana V. (Russian Academy of Sciences) Date 2017 Abstract Brine samples from Kulunda Steppe soda lakes (Altai, Russia) were inoculated into a hypersaline alkaline mineral medium with β-keratin (chicken feather) as a substrate. The micro-organisms dominating the enrichment culture were isolated by limiting serial dilution on the same medium with casein as a substrate. The cells of strain BSker1T were motile, curved rods. The strain was an obligately aerobic heterotroph utilizing proteins and peptides as growth substrates. The isolate was an obligate alkaliphile with a pH range for growth from pH 8.5 to 10.25 (optimum at pH 9.5), and it was extremely salt tolerant, growing with between 1 and 4.5 M total Na+ (optimally at 2–2.5 M). BSker1T had a unique composition of polar lipid fatty acids, dominated by two C17 species. The membrane polar lipids included multiple unidentified phospholipids and two aminolipids. According to phylogenetic analysis of the 16S rRNA gene sequence, the isolate forms a novel branch within the family Ectothiorhodospiraceae (class Gammaproteobacteria) with the highest sequence similarity to the members of this family being 91 %. On the basis of distinct phenotypic and genotypic properties, strain BSker1T (=JCM 31341T=UNIQEM U1008T) is proposed to be classified as a representative of a novel genus and species, Natronospira proteinivora gen. nov., sp. nov. Subject Aerobic proteolyticsGammaproteobacteriaHaloalkaliphilicSoda lakes To reference this document use: http://resolver.tudelft.nl/uuid:d0ad785a-dc29-4944-a636-a18dabe3ac01 DOI https://doi.org/10.1099/ijsem.0.001983 Embargo date 2018-07-31 ISSN 1466-5026 Source International Journal of Systematic and Evolutionary Microbiology, 67 (8), 2604-2608 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2017 Dimitry Y. Sorokin, Ilya V. Kublanov, Tatiana V. Khijniak Files PDF Natronotalea017.pdf 953.17 KB Close viewer /islandora/object/uuid:d0ad785a-dc29-4944-a636-a18dabe3ac01/datastream/OBJ/view