Print Email Facebook Twitter Improvement on bacteria-induced calcium mineralization ability of Bacillus pseudofirmus by an integrated high-throughput screening strategy Title Improvement on bacteria-induced calcium mineralization ability of Bacillus pseudofirmus by an integrated high-throughput screening strategy Author Zhang, J.L. Deng, X. Feng, X. Han, N.X. Jonkers, H.M. Faculty Civil Engineering and Geosciences Department Structural Engineering Date 2013-06-16 Abstract The CaCO3-mineralizing bacteria from different taxonomic groups have shown potential in restoration of construction material such as concretes, cements and stony materials. However, these strains are far from the demand of practical application due to some shortages, including the low mineralizing capacity. In this work, an integrated high-throughput screening(HTS) strategy was developed in an attempt to screen enhanced CaCO3-producing mutants of Bacillus pseudofirmus. The isolates, mutagenized by ultraviolet radiation (UV), were cultivated in the suitable media containing calcium ion by using 96-deep-well microliter plates. The residual calcium in supernatants from micro-cultivation plates was determined by O-Cresolphthalein Complexone method to evaluate the CaCO3-producing activity. On the other hand, the activity of carbonic anhydrase of the isolates, which is responsible for the formation of CO3 2-, was also monitored to further evidence the bacteriainduced calcium mineralization process. As a result, the mutant strain B388 with the highest calcium mineralizing capability (92.67%, 11.1% higher than that of the original strain) was obtained from about 3000 isolates. It is proved that this novel HTS strategy is a promising procedure for selecting high efficient calcium mineralizing microorganisms. Subject biomineralizationBacillus pseudofirmusUV-induced Mutationhigh throughput screening To reference this document use: http://resolver.tudelft.nl/uuid:d18d7db2-d535-443c-aa63-44d6a93e429d Publisher Magnel Laboratory for Concrete Research ISBN 9789082073713 Source uuid:3078d20c-2173-4c13-a4d7-106207c78577 Source ICSHM 2013: Proceedings of the 4th International Conference on Self-Healing Materials, Ghent, Belgium, 16-20 June 2013 Part of collection Institutional Repository Document type conference paper Rights (c) 2013 The Author(s) Files PDF Jonkers_2013.pdf 544.96 KB Close viewer /islandora/object/uuid:d18d7db2-d535-443c-aa63-44d6a93e429d/datastream/OBJ/view