Print Email Facebook Twitter Electroporation of biomimetic vesicles Title Electroporation of biomimetic vesicles Author Perrier, D.L. (TU Delft Reservoir Engineering) Contributor Kreutzer, M.T. (promotor) Boukany, P. (copromotor) Degree granting institution Delft University of Technology Date 2018-11-26 Abstract Electroporation is a popular technique to permeabilize the membrane for different purposes such as medical treatments, food processing and biomass processing. In this thesis, we use the bottom-up approach to unravel the role of specific cellular components in the electroporation of cellular membranes. We have studied the role of the gel-phase domains in the membrane and the contribution of the actin-cortex during electroporation. In order to do so, we have prepared binary-phase vesicles, containing fluid- and gelphase lipids, and actin-cortex encapsulated vesicles. Consequently, the electroporation mechanisms of these two samples provide systematic insight in the electroporation mechanism of a single cell. Subject electroporationelectropermeabilizationelectric field, lipid vesiclesgiant unilamellar vesiclesGUVsgel-phase lipidsfluid-phase lipidsbinary-phaseactin network To reference this document use: https://doi.org/10.4233/uuid:dd37171a-f008-4874-9616-fb50367b8089 ISBN 978.90.8593.371.7 Part of collection Institutional Repository Document type doctoral thesis Rights © 2018 D.L. Perrier Files PDF 02566_INN_FC_V3.pdf 12.62 MB Close viewer /islandora/object/uuid:dd37171a-f008-4874-9616-fb50367b8089/datastream/OBJ/view