Focused ultrasound neuromodulation on a multiwell MEA

Title

Focused ultrasound neuromodulation on a multiwell MEA

 Author

Saccher, M. (TU Delft Electronic Components, Technology and Materials) ORCID 0000-0002-9509-7815
Kawasaki, S. (TU Delft Electronic Components, Technology and Materials)
Proietti Onori, Martina (Erasmus MC)
van Woerden, Geeske M. (Erasmus MC)
Giagka, Vasiliki (TU Delft Bio-Electronics; Fraunhofer Institute for Reliability and Microintegration IZM) ORCID 0000-0002-0822-2121
Dekker, R. (TU Delft Electronic Components, Technology and Materials; Philips Research) ORCID 0000-0003-0839-4342

 Date

2022

 Abstract

Background
Microelectrode arrays (MEA) enable the measurement and stimulation of the electrical activity of cultured cells. The integration of other neuromodulation methods will significantly enhance the application range of MEAs to study their effects on neurons. A neuromodulation method that is recently gaining more attention is focused ultrasound neuromodulation (FUS), which has the potential to treat neurological disorders reversibly and precisely.

Methods
In this work, we present the integration of a focused ultrasound delivery system with a multiwell MEA plate.

Results
The ultrasound delivery system was characterised by ultrasound pressure measurements, and the integration with the MEA plate was modelled with finite-element simulations of acoustic field parameters. The results of the simulations were validated with experimental visualisation of the ultrasound field with Schlieren imaging. In addition, the system was tested on a murine primary hippocampal neuron culture, showing that ultrasound can influence the activity of the neurons.

Conclusions
Our system was demonstrated to be suitable for studying the effect of focused ultrasound on neuronal cultures. The system allows reproducible experiments across the wells due to its robustness and simplicity of operation.

 Subject

OA-Fund TU Delft

 To reference this document use:

http://resolver.tudelft.nl/uuid:dfdeb7da-6405-4adc-8a79-3ff7d82af230

 DOI

https://doi.org/10.1186/s42234-021-00083-7

 ISSN

2332-8886

 Source

Bioelectronic Medicine, 8 (1)

 Part of collection

Institutional Repository

 Document type

journal article

 Rights

© 2022 M. Saccher, S. Kawasaki, Martina Proietti Onori, Geeske M. van Woerden, Vasiliki Giagka, R. Dekker
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