Print Email Facebook Twitter An Efficient Strategy for the Production of Epoxidized Oils Title An Efficient Strategy for the Production of Epoxidized Oils: Natural Deep Eutectic Solvent-Based Enzymatic Epoxidation Author Zhang, Tianyu (South China University of Technology) Ma, Yunjian (South China University of Technology) Tan, Chin Ping (Universiti Putra Malaysia) Hollmann, F. (TU Delft BT/Biocatalysis) Wang, Jianrong (South China University of Technology) Yang, Bo (South China University of Technology) Wang, Yonghua (South China University of Technology) Date 2019 Abstract Poor H 2 O 2 -resistance by enzymes is a key bottleneck in the epoxidation process of oil by enzymatic methods. In this study, the stability of three lipases, from Aspergillus oryzae lipase (AOL), Aspergillus fumigatus lipase B (AflB), and marine Janibacter (MAJ1), in the presence of H 2 O 2 was evaluated in different types of natural deep eutectic solvents (NADES). This stability was strengthened significantly in the NADES compared to the buffer. Specifically, AOL retained 84.7% of its initial activity in the presence of choline chloride/sorbitol (1:1 M ratio) and 3 mol L −1 H 2 O 2 after 24 h incubation at 40°C. In addition, the two-phase epoxidation process was optimized with AOL in ChCl/sorbitol to reach up to 96.8% conversion under the optimized conditions (molar ratio of octanoic acid/H 2 O 2 /C=C-bonds = 0.3:1.5:1, enzyme loading of 15 U g −1 of soybean oil, ChCl/sorbitol content of 70.0% of the weight of hydrophilic phase, and reaction temperature of 50°C). Moreover, the lipase dispersed in NADES retained approximately 66% of its initial activity after being used for seven batch cycles. Overall, NADES-based enzymatic epoxidation is a feasible and promising strategy for the synthesis of epoxidized oils. Subject Enzyme catalysisEpoxidationLipaseNatural deep eutectic solventSoybean oil To reference this document use: http://resolver.tudelft.nl/uuid:e27fa935-b576-4eec-826f-050499979d79 DOI https://doi.org/10.1002/aocs.12220 Embargo date 2019-09-20 ISSN 0003-021X Source JAOCS: Journal of the American Oil Chemists' Society, 96 (6), 671-679 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2019 Tianyu Zhang, Yunjian Ma, Chin Ping Tan, F. Hollmann, Jianrong Wang, Bo Yang, Yonghua Wang Files PDF Zhang_et_al_2019_Journal_ ... ociety.pdf 1.58 MB Close viewer /islandora/object/uuid:e27fa935-b576-4eec-826f-050499979d79/datastream/OBJ/view