Print Email Facebook Twitter Stoichiometry and kinetics of single and mixed substrate uptake in Aspergillus niger Title Stoichiometry and kinetics of single and mixed substrate uptake in Aspergillus niger Author da Fonte Lameiras, F. (TU Delft OLD BT/Cell Systems Engineering) Ras, C. (TU Delft OLD BT/Cell Systems Engineering) ten Pierick, A. (TU Delft OLD BT/Cell Systems Engineering) Heijnen, J.J. (TU Delft OLD BT/Cell Systems Engineering) van Gulik, W.M. (TU Delft OLD BT/Cell Systems Engineering) Date 2017-10-19 Abstract In its natural environment, the filamentous fungus Aspergillus niger grows on decaying fruits and plant material, thereby enzymatically degrading the lignocellulosic constituents (lignin, cellulose, hemicellulose, and pectin) into a mixture of mono- and oligosaccharides. To investigate the kinetics and stoichiometry of growth of this fungus on lignocellulosic sugars, we carried out batch cultivations on six representative monosaccharides (glucose, xylose, mannose, rhamnose, arabinose, and galacturonic acid) and a mixture of these. Growth on these substrates was characterized in terms of biomass yields, oxygen/biomass ratios, and specific conversion rates. Interestingly, in combination, some of the carbon sources were consumed simultaneously and some sequentially. With a previously developed protocol, a sequential chemostat cultivation experiment was performed on a feed mixture of the six substrates. We found that the uptake of glucose, xylose, and mannose could be described with a Michaelis–Menten-type kinetics; however, these carbon sources seem to be competing for the same transport systems, while the uptake of arabinose, galacturonic acid, and rhamnose appeared to be repressed by the presence of other substrates. Subject Aspergillus nigerKineticsPlant waste streamsStoichiometrySubstrate uptake To reference this document use: http://resolver.tudelft.nl/uuid:e2ecf3aa-ea71-41f1-a1c3-a51ca6b4d9ff DOI https://doi.org/10.1007/s00449-017-1854-3 ISSN 1615-7591 Source Bioprocess and Biosystems Engineering: bioreactors, upstream and downstream processes, measurement and control, 1-14 Part of collection Institutional Repository Document type journal article Rights © 2017 F. da Fonte Lameiras, C. Ras, A. ten Pierick, J.J. Heijnen, W.M. van Gulik Files PDF 10.1007_s00449_017_1854_3.pdf 1.66 MB Close viewer /islandora/object/uuid:e2ecf3aa-ea71-41f1-a1c3-a51ca6b4d9ff/datastream/OBJ/view