Print Email Facebook Twitter Numerical and experimental trapping analysis of simple and cost-effective acoustic tweezers Title Numerical and experimental trapping analysis of simple and cost-effective acoustic tweezers Author Tsan, Vi-hung (TU Delft Mechanical, Maritime and Materials Engineering) Contributor Smith, C.S. (mentor) Verbiest, G.J. (mentor) Fan, D. (mentor) Degree granting institution Delft University of Technology Programme Mechanical Engineering | Systems and Control Date 2022-09-14 Abstract Acoustic Force Spectroscopy (AFS) is a versatile tool that uses sound waves to manipulate tiny particles such as cells, bacteria, and even zebrafish embryos in microfluidic systems. This kind of acoustic tweezer is gaining increasing attention due to its high throughput capability and non-invasiveness. In addition, this device allows for parallel manipulation of bio-molecules. Therefore, it can provide statistically significant data about the DNA replication process, which is widely considered a stochastic process. Understanding the dynamics of the DNA replication process plays a vital role in developing medicine to cure diseases that are still incurable today. Most single-molecule techniques, such as optical tweezers, magnetic tweezers, and atomic force microscopy, can only manipulate a limited number of particles simultaneously. Therefore, obtaining statistically significant data with these methods is laborious, time-consuming, and expensive. This report describes the design, fabrication, simulation and characterization of an easy-tobuild and cost-effective acoustic tweezer. The trapping stiffness of this device is derived from finite element modelling and experiments. This property is used to benchmark the acoustic tweezer developed in this project against other microparticle manipulation devices found in the literature. The results show that the acoustic tweezer developed in this project provides sufficient trap stiffness for studying DNA replication. Subject Acoustic trappinglow-costmicrofluidicsacoustic force spectroscopy To reference this document use: http://resolver.tudelft.nl/uuid:e61fcf92-75b8-4cde-9862-f7b49e850290 Part of collection Student theses Document type master thesis Rights © 2022 Vi-hung Tsan Files PDF Msc_thesis_final_report_7_.pdf 40.86 MB Close viewer /islandora/object/uuid:e61fcf92-75b8-4cde-9862-f7b49e850290/datastream/OBJ/view