Proteomic analysis of methanonatronarchaeum thermophilum AMET1, a representative of a putative new class of euryarchaeota, “methanonatronarchaeia”

Proteomic analysis of methanonatronarchaeum thermophilum AMET1, a representative of a putative new class of euryarchaeota, “methanonatronarchaeia”

Ferrer, Manuel (University of the Balearic Islands)
Sorokin, Dimitry Y. (TU Delft BT/Environmental Biotechnology; Russian Academy of Sciences)
Wolf, Yuri I. (National Library of Medicine)
Ciordia, Sergio (University of the Balearic Islands)
Mena, María Carmen (University of the Balearic Islands)
Bargiela, Rafael (University of the Balearic Islands)
Koonin, Eugene V. (National Library of Medicine)
Makarova, Kira S. (National Library of Medicine)

2018

The recently discovered Methanonatronarchaeia are extremely halophilic and moderately thermophilic methyl-reducing methanogens representing a novel class-level lineage in the phylum Euryarchaeota related to the class Halobacteria. Here we present a detailed analysis of 1D-nano liquid chromatography–electrospray ionization tandem mass spectrometry data obtained for “Methanonatronarchaeum thermophilum” AMET1 grown in different physiological conditions, including variation of the growth temperature and substrates. Analysis of these data allows us to refine the current understanding of the key biosynthetic pathways of this triple extremophilic methanogenic euryarchaeon and identify proteins that are likely to be involved in its response to growth condition changes.

Genomics
Halophiles
Methanogenesis
Methanonatronarchaeia
Proteomics

http://resolver.tudelft.nl/uuid:e7b7eac6-4a2f-4439-bf56-b3e73698664a

https://doi.org/10.3390/genes9020028

2073-4425

Genes, 9 (2)

Institutional Repository

journal article

© 2018 Manuel Ferrer, Dimitry Y. Sorokin, Yuri I. Wolf, Sergio Ciordia, María Carmen Mena, Rafael Bargiela, Eugene V. Koonin, Kira S. Makarova