Print Email Facebook Twitter Lipid recovery from a vegetable oil emulsion using microbial enrichment cultures Title Lipid recovery from a vegetable oil emulsion using microbial enrichment cultures Author Tamis, J. Sorokin, D.Y. Jiang, Y. Van Loosdrecht, M.C.M. Kleerebezem, R. Faculty Applied Sciences Department BT/Biotechnology Date 2015-03-10 Abstract Background Many waste streams have a relatively high vegetable oil content, which is a potential resource that should be recovered. Microbial storage compound production for the recovery of lipids from lipid-water emulsions with open (unsterilized) microbial cultures was investigated in a sequencing batch reactor using a diluted vegetable oil emulsion as model substrate. Results After feeding, triacylglycerides (TAG) were accumulated intracellular by the microbial enrichment culture and subsequently used for growth in the remainder of the sequencing batch cycle. Roughly 50% of the added TAG could be recovered as intracellular lipids in this culture. The maximum lipid storage capacity of the enrichment culture was 54% on volatile suspended solids (VSS) mass basis in a separate fed-batch accumulation experiment. The microbial community was dominated by a lipolytic fungus, Trichosporon gracile, that was responsible for intracellular lipid accumulation but also a significant fraction of lipolytic and long chain fatty-acid-utilizing bacteria was present. Conclusion Herewith, we demonstrate an effective strategy for enrichment of a microbial community that can accumulate significant amounts of lipids from wastewaters without the need for sterilization of substrates or equipment. Further optimization of this process will make recovery of lipids from wastewater possible. Subject vegetable oilstorage compoundsmicrobial enrichment culturefeast-famineresource recoveryOA-Fund TU Delft To reference this document use: http://resolver.tudelft.nl/uuid:e7e53762-55a7-4017-8d1e-3ea6ec649471 DOI https://doi.org/10.1186/s13068-015-0228-9 Publisher BioMed Central ISSN 1754-6834 Source http://www.biotechnologyforbiofuels.com/content/8/1/39 Source Biotechnology for Biofuels, 8, 2015 Part of collection Institutional Repository Document type journal article Rights © 2015 The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. Files PDF Tamis_2015.pdf 2.41 MB Close viewer /islandora/object/uuid:e7e53762-55a7-4017-8d1e-3ea6ec649471/datastream/OBJ/view