Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy

Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy

Pozzi, P. (TU Delft Team Raf Van de Plas)
Wilding, D. (TU Delft Team Raf Van de Plas)
Soloviev, O.A. (TU Delft Team Raf Van de Plas; Flexible Optical B.V.; ITMO University)
Vdovin, Gleb (TU Delft Team Raf Van de Plas; Flexible Optical B.V.; ITMO University)
Verhaegen, M.H.G. (TU Delft Team Raf Van de Plas)

Douglass, Michael R. (editor)
Lee, Benjamin L. (editor)

2018

In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and scanning. A binary DMD is positioned in an image plane of the detection optical path, acting as a dynamic array of reflective confocal pinholes, images by a high performance CMOS camera. A second camera detects images of the light rejected by the pinholes for sensorless aberration correction.

Adaptive optics
Confocal microscopy
Digital micromirror devices

http://resolver.tudelft.nl/uuid:e8f8fdc5-37b6-4a58-bffb-3f8219c35ae8

https://doi.org/10.1117/12.2288436

SPIE, Bellingham, WA, USA

978-1-510615779

Proceedings of SPIE: Emerging Digital Micromirror Device Based Systems and Applications X, 10546

Emerging Digital Micromirror Device Based Systems and Applications X, 2018-01-29 → 2018-01-30, San Francisco, United States

Proceedings of SPIE, 1605-7422, 10546

Institutional Repository

conference paper

© 2018 P. Pozzi, D. Wilding, O.A. Soloviev, Gleb Vdovin, M.H.G. Verhaegen