Print Email Facebook Twitter Ammonium-assimilating microbiome Title Ammonium-assimilating microbiome: A halophilic biosystem rationally optimized by carbon to nitrogen ratios with stable nitrogen conversion and microbial structure Author Zhang, Mengru (Shandong University) Han, Fei (Shandong University) Liu, Zhe (Shandong University) Han, Yufei (Shandong University) Li, L. (TU Delft Sanitary Engineering) Zhou, Weizhi (Shandong University) Date 2022 Abstract The contradiction between theoretical metabolism of ammonium assimilation and experiential understanding of conventional biosystems makes the rational optimization of the ammonium-assimilating microbiome through carbon to nitrogen (C/N) ratios perplexing. The effect of different C/N ratios on ammonium-assimilating biosystems was investigated in saline wastewater treatment. C/N ratios significantly hindered the nutrient removal efficiency, but ammonium-assimilating biosystems maintained functional stability in nitrogen conversions and microbial communities. With sufficient biomass, higher than 86% ammonium and 73% phosphorus were removed when C/N ratios were higher than 25. Ammonium assimilation dominated the nitrogen metabolism in all biosystems even under relatively low C/N ratios, evidenced by the extremely low abundances of nitrification functional genes. Different C/N ratios did not significantly change the bacterial community structure of ammonium-assimilating biosystems. It is anticipated that the ammonium-assimilating biosystem with advantages of clear metabolic pathway and easy optimization can be applied to nutrient removal and recovery in saline environments. Subject Ammonium assimilationAmmonium-assimilating biosystemsBacterial communityC/N ratiosSaline wastewater treatment To reference this document use: http://resolver.tudelft.nl/uuid:ee504601-79bb-48ee-97bc-bb5d54374695 DOI https://doi.org/10.1016/j.biortech.2022.126911 Embargo date 2023-07-01 ISSN 0960-8524 Source Bioresource Technology, 350 Bibliographical note Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2022 Mengru Zhang, Fei Han, Zhe Liu, Yufei Han, L. Li, Weizhi Zhou Files PDF 1_s2.0_S0960852422002401_main.pdf 6.31 MB Close viewer /islandora/object/uuid:ee504601-79bb-48ee-97bc-bb5d54374695/datastream/OBJ/view