Print Email Facebook Twitter EPR spectroscopy of complex biological iron–sulfur systems Title EPR spectroscopy of complex biological iron–sulfur systems Author Hagen, W.R. (TU Delft BT/Biocatalysis) Date 2018 Abstract From the very first discovery of biological iron–sulfur clusters with EPR, the spectroscopy has been used to study not only purified proteins but also complex systems such as respiratory complexes, membrane particles and, later, whole cells. In recent times, the emphasis of iron–sulfur biochemistry has moved from characterization of individual proteins to the systems biology of iron–sulfur biosynthesis, regulation, degradation, and implications for human health. Although this move would suggest a blossoming of System-EPR as a specific, non-invasive monitor of Fe/S (dys)homeostasis in whole cells, a review of the literature reveals limited success possibly due to technical difficulties in adherence to EPR spectroscopic and biochemical standards. In an attempt to boost application of System-EPR the required boundary conditions and their practical applications are explicitly and comprehensively formulated. Subject EPRIron–sulfurSystems biology To reference this document use: http://resolver.tudelft.nl/uuid:fab2cea7-40fd-4e5d-8250-c8c80c6dad81 DOI https://doi.org/10.1007/s00775-018-1543-y ISSN 0949-8257 Source Journal of Biological Inorganic Chemistry, 1-12 Part of collection Institutional Repository Document type journal article Rights © 2018 W.R. Hagen Files PDF 10.1007_s00775_018_1543_y.pdf 1.69 MB Close viewer /islandora/object/uuid:fab2cea7-40fd-4e5d-8250-c8c80c6dad81/datastream/OBJ/view