Print Email Facebook Twitter Purkinje Cell Activity Resonation Generates Rhythmic Behaviors at the Preferred Frequency of 8 Hz Title Purkinje Cell Activity Resonation Generates Rhythmic Behaviors at the Preferred Frequency of 8 Hz Author Bauer, Staf (Erasmus MC) van Wingerden, Nathalie (Erasmus MC) Jacobs, Thomas (Erasmus MC) van der Horst, Annabel (Erasmus MC) Zhai, Peipei (Erasmus MC) Betting, Jan Harm L.F. (Erasmus MC) Strydis, C. (TU Delft Computer Engineering; TU Delft Bio-Electronics; Erasmus MC) White, Joshua J. (Erasmus MC) De Zeeuw, Chris I. (Erasmus MC; Netherlands Institute for Neuroscience NIN - KNAW) Romano, Vincenzo (Erasmus MC) Date 2022 Abstract Neural activity exhibits oscillations, bursts, and resonance, enhancing responsiveness at preferential frequencies. For example, theta-frequency bursting and resonance in granule cells facilitate synaptic transmission and plasticity mechanisms at the input stage of the cerebellar cortex. However, whether theta-frequency bursting of Purkinje cells is involved in generating rhythmic behavior has remained neglected. We recorded and optogenetically modulated the simple and complex spike activity of Purkinje cells while monitoring whisker movements with a high-speed camera of awake, head-fixed mice. During spontaneous whisking, both simple spike activity and whisker movement exhibit peaks within the theta band. Eliciting either simple or complex spikes at frequencies ranging from 0.5 to 28 Hz, we found that 8 Hz is the preferred frequency around which the largest movement is induced. Interestingly, oscillatory whisker movements at 8 Hz were also generated when simple spike bursting was induced at 2 and 4 Hz, but never via climbing fiber stimulation. These results indicate that 8 Hz is the resonant frequency at which the cerebellar-whisker circuitry produces rhythmic whisking. Subject cerebellumoptogenetics stimulationPurkinje celltheta resonancewhisker To reference this document use: http://resolver.tudelft.nl/uuid:1223280c-b08f-4de0-ae54-e1ef75db77e0 DOI https://doi.org/10.3390/biomedicines10081831 Source Biomedicines, 10 (8) Part of collection Institutional Repository Document type journal article Rights © 2022 Staf Bauer, Nathalie van Wingerden, Thomas Jacobs, Annabel van der Horst, Peipei Zhai, Jan Harm L.F. Betting, C. Strydis, Joshua J. White, Chris I. De Zeeuw, Vincenzo Romano Files PDF biomedicines_10_01831_v3.pdf 1.61 MB Close viewer /islandora/object/uuid:1223280c-b08f-4de0-ae54-e1ef75db77e0/datastream/OBJ/view