Print Email Facebook Twitter Label-Free Optical Detection of DNA Translocations through Plasmonic Nanopores Title Label-Free Optical Detection of DNA Translocations through Plasmonic Nanopores Author Verschueren, D.V. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Pud, S. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Shi, X. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft; East China University of Science and Technology) de Angelis, L. (TU Delft QN/Kuipers Lab) Kuipers, L. (TU Delft QN/Quantum Nanoscience) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Department QN/Quantum Nanoscience Date 2018 Abstract Solid-state nanopores are single-molecule sensors that hold great potential for rapid protein and nucleic-acid analysis. Despite their many opportunities, the conventional ionic current detection scheme that is at the heart of the sensor suffers inherent limitations. This scheme intrinsically couples signal strength to the driving voltage, requires the use of high-concentration electrolytes, suffers from capacitive noise, and impairs high-density sensor integration. Here, we propose a fundamentally different detection scheme based on the enhanced light transmission through a plasmonic nanopore. We demonstrate that translocations of single DNA molecules can be optically detected, without the need of any labeling, in the transmitted light intensity through an inverted-bowtie plasmonic nanopore. Characterization and the cross-correlation of the optical signals with their electrical counterparts verify the plasmonic basis of the optical signal. We demonstrate DNA translocation event detection in a regime of driving voltages and buffer conditions where traditional ionic current sensing fails. This label-free optical detection scheme offers opportunities to probe native DNA-protein interactions at physiological conditions. Subject DNA translocationoptical transmissionplasmon resonance sensingplasmonic nanoporessolid-state nanopores To reference this document use: http://resolver.tudelft.nl/uuid:2a2ae475-c406-4c04-81ae-b7bfa502d30f DOI https://doi.org/10.1021/acsnano.8b06758 ISSN 1936-0851 Source ACS Nano (online) Part of collection Institutional Repository Document type journal article Rights © 2018 D.V. Verschueren, S. Pud, X. Shi, L. de Angelis, L. Kuipers, C. Dekker Files PDF acsnano.8b06758.pdf 1.98 MB Close viewer /islandora/object/uuid:2a2ae475-c406-4c04-81ae-b7bfa502d30f/datastream/OBJ/view