Print Email Facebook Twitter Nanodiamonds as multi-purpose labels for microscopy Title Nanodiamonds as multi-purpose labels for microscopy Author Hemelaar, S. R. (Rijksuniversiteit Groningen) de Boer, P. (Rijksuniversiteit Groningen) Chipaux, M. (Rijksuniversiteit Groningen) Zuidema, W. (TU Delft ImPhys/Charged Particle Optics) Hamoh, T. (Rijksuniversiteit Groningen) Perona Martinez, F. (Rijksuniversiteit Groningen) Nagl, A. (Rijksuniversiteit Groningen) Hoogenboom, J.P. (TU Delft ImPhys/Charged Particle Optics) Giepmans, B. N.G. (Rijksuniversiteit Groningen) Schirhagl, R. (Rijksuniversiteit Groningen) Date 2017 Abstract Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility to excite fluorescence using a focused electron beam (cathodoluminescence; CL) for high-resolution localization; and (iii) the potential use for nanoscale sensing. For all these schemes, the development of versatile molecular labeling using relatively small diamonds is essential. Here, we show the direct targeting of a biological molecule with nanodiamonds as small as 70 nm using a streptavidin conjugation and standard antibody labelling approach. We also show internalization of 40 nm sized nanodiamonds. The fluorescence from the nanodiamonds survives osmium-fixation and plastic embedding making them suited for correlative light and electron microscopy. We show that CL can be observed from epon-embedded nanodiamonds, while surface-exposed nanoparticles also stand out in secondary electron (SE) signal due to the exceptionally high diamond SE yield. Finally, we demonstrate the magnetic read-out using fluorescence from diamonds prior to embedding. Thus, our results firmly establish nanodiamonds containing nitrogen-vacancy centers as unique, versatile probes for combining and correlating different types of microscopy, from fluorescence imaging and magnetometry to ultrastructural investigation using electron microscopy. Subject imaging and sensingimaging techniques To reference this document use: http://resolver.tudelft.nl/uuid:2a94a47b-8c2c-4aab-809e-0d84589f24f7 DOI https://doi.org/10.1038/s41598-017-00797-2 ISSN 2045-2322 Source Scientific Reports, 7 (1) Part of collection Institutional Repository Document type journal article Rights © 2017 S. R. Hemelaar, P. de Boer, M. Chipaux, W. Zuidema, T. Hamoh, F. Perona Martinez, A. Nagl, J.P. Hoogenboom, B. N.G. Giepmans, R. Schirhagl Files PDF s41598_017_00797_2.pdf 2.55 MB Close viewer /islandora/object/uuid:2a94a47b-8c2c-4aab-809e-0d84589f24f7/datastream/OBJ/view