Print Email Facebook Twitter Directing Min protein patterns with advective bulk flow Title Directing Min protein patterns with advective bulk flow Author Meindlhumer, S. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Brauns, Fridtjof (Ludwig Maximilians University) Finžgar, Jernej Rudi (Ludwig Maximilians University) Kerssemakers, J.W.J. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Frey, Erwin (Ludwig Maximilians University; Max Planck School Matter to Life, Munich) Date 2023 Abstract The Min proteins constitute the best-studied model system for pattern formation in cell biology. We theoretically predict and experimentally show that the propagation direction of in vitro Min protein patterns can be controlled by a hydrodynamic flow of the bulk solution. We find downstream propagation of Min wave patterns for low MinE:MinD concentration ratios, upstream propagation for large ratios, but multistability of both propagation directions in between. Whereas downstream propagation can be described by a minimal model that disregards MinE conformational switching, upstream propagation can be reproduced by a reduced switch model, where increased MinD bulk concentrations on the upstream side promote protein attachment. Our study demonstrates that a differential flow, where bulk flow advects protein concentrations in the bulk, but not on the surface, can control surface-pattern propagation. This suggests that flow can be used to probe molecular features and to constrain mathematical models for pattern-forming systems. To reference this document use: http://resolver.tudelft.nl/uuid:37e3d82e-2bc7-4b92-bad0-320d931af6a8 DOI https://doi.org/10.1038/s41467-023-35997-0 ISSN 2041-1723 Source Nature Communications, 14 (1) Part of collection Institutional Repository Document type journal article Rights © 2023 S. Meindlhumer, Fridtjof Brauns, Jernej Rudi Finžgar, J.W.J. Kerssemakers, C. Dekker, Erwin Frey Files PDF s41467_023_35997_0.pdf 2.55 MB Close viewer /islandora/object/uuid:37e3d82e-2bc7-4b92-bad0-320d931af6a8/datastream/OBJ/view