Print Email Facebook Twitter Fighting Antibiotic-Resistant Bacterial Infections by Surface Biofunctionalization of 3D-Printed Porous Titanium Implants with Reduced Graphene Oxide and Silver Nanoparticles Title Fighting Antibiotic-Resistant Bacterial Infections by Surface Biofunctionalization of 3D-Printed Porous Titanium Implants with Reduced Graphene Oxide and Silver Nanoparticles Author San, H. (TU Delft Biomaterials & Tissue Biomechanics; Henan Polytechnic University) Paresoglou, M. (Student TU Delft) Minneboo, M.B. (TU Delft Biomaterials & Tissue Biomechanics) van Hengel, I.A.J. (TU Delft Biomaterials & Tissue Biomechanics) Yilmaz, A. (TU Delft Team Yaiza Gonzalez Garcia) Gonzalez Garcia, Y. (TU Delft Team Yaiza Gonzalez Garcia) Fluit, Ad C. (University Medical Center Utrecht) Hagedoorn, P.L. (TU Delft BT/Biocatalysis) Fratila-Apachitei, E.L. (TU Delft Biomaterials & Tissue Biomechanics) Apachitei, I. (TU Delft Biomaterials & Tissue Biomechanics) Zadpoor, A.A. (TU Delft Biomaterials & Tissue Biomechanics) Date 2022 Abstract Nanoparticles (NPs) have high multifunctional potential to simultaneously enhance implant osseointegration and prevent infections caused by antibiotic-resistant bacteria. Here, we present the first report on using plasma electrolytic oxidation (PEO) to incorporate different combinations of reduced graphene oxide (rGO) and silver (Ag) NPs on additively manufactured geometrically ordered volume-porous titanium implants. The rGO nanosheets were mainly embedded parallel with the PEO surfaces. However, the formation of ‘nano-knife’ structures (particles embedded perpendicularly to the implant surfaces) was also found around the pores of the PEO layers. Enhanced in vitro antibacterial activity against methicillin-resistant Staphylococcus aureus was observed for the rGO+Ag-containing surfaces compared to the PEO surfaces prepared only with AgNPs. This was caused by a significant improvement in the generation of reactive oxygen species, higher levels of Ag+ release, and the presence of rGO ‘nano-knife’ structures. In addition, the implants developed in this study stimulated the metabolic activity and osteogenic differentiation of MC3T3-E1 preosteoblast cells compared to the PEO surfaces without nanoparticles. Therefore, the PEO titanium surfaces incorporating controlled levels of rGO+Ag nanoparticles have high clinical potential as multifunctional surfaces for 3D-printed orthopaedic implants. Subject antibiotic-resistant bacteriareduced graphene oxidesilver nanoparticlesplasma electrolytic oxidationimplant-associated infectionstitanium3D printing To reference this document use: http://resolver.tudelft.nl/uuid:3ce073e6-71c1-4b3e-9253-11ebd7ad21ee DOI https://doi.org/10.3390/ijms23169204 ISSN 1422-0067 Source International Journal of Molecular Sciences (Online), 23 (16) Part of collection Institutional Repository Document type journal article Rights © 2022 H. San, M. Paresoglou, M.B. Minneboo, I.A.J. van Hengel, A. Yilmaz, Y. Gonzalez Garcia, Ad C. Fluit, P.L. Hagedoorn, E.L. Fratila-Apachitei, I. Apachitei, A.A. Zadpoor Files PDF ijms_23_09204.pdf 8.41 MB Close viewer /islandora/object/uuid:3ce073e6-71c1-4b3e-9253-11ebd7ad21ee/datastream/OBJ/view