Combined optical sizing and acoustical characterization of single freely-floating microbubbles

Combined optical sizing and acoustical characterization of single freely-floating microbubbles

Luan, Ying (Erasmus MC)
Renaud, Guillaume (Erasmus MC; UPMC-Sorbonne Universités & CNRS)
Raymond, Jason L. (Erasmus MC)
Segers, Tim (University of Twente)
Lajoinie, Guillaume (University of Twente)
Beurskens, Robert (Erasmus MC)
Mastik, Frits (Erasmus MC)
Kokhuis, Tom J A (Erasmus MC)
van der Steen, A.F.W. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC)
Versluis, Michel (University of Twente)
de Jong, N. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC)

2016-12-07

In this study we present a combined optical sizing and acoustical characterization technique for the study of the dynamics of single freely-floating ultrasound contrast agent microbubbles exposed to long burst ultrasound excitations up to the milliseconds range. A co-axial flow device was used to position individual microbubbles on a streamline within the confocal region of three ultrasound transducers and a high-resolution microscope objective. Bright-field images of microbubbles passing through the confocal region were captured using a high-speed camera synchronized to the acoustical data acquisition to assess the microbubble response to a 1-MHz ultrasound burst. Nonlinear bubble vibrations were identified at a driving pressure as low as 50 kPa. The results demonstrate good agreement with numerical simulations based on the shell-buckling model proposed by Marmottant et al. [J. Acoust. Soc. Am. 118, 3499-3505 (2005)]. The system demonstrates the potential for a high-throughput in vitro characterization of individual microbubbles.

Ultranonography
Bubble dynamics
Nonlinear dynamics
Vibration testing
Cameras

http://resolver.tudelft.nl/uuid:47632dfc-4c6a-4860-8a7b-d803d5bc6395

https://doi.org/10.1063/1.4971391

2017-12-07

0003-6951

Applied Physics Letters, 109 (23)

Institutional Repository

journal article

© 2016 Ying Luan, Guillaume Renaud, Jason L. Raymond, Tim Segers, Guillaume Lajoinie, Robert Beurskens, Frits Mastik, Tom J A Kokhuis, A.F.W. van der Steen, Michel Versluis, N. de Jong