Point spread function based image reconstruction in optical projection tomography

Point spread function based image reconstruction in optical projection tomography

Trull, A.K. (TU Delft ImPhys/Quantitative Imaging)
van der Horst, J. (TU Delft ImPhys/Quantitative Imaging)
Palenstijn, W.J. (Centrum Wiskunde & Informatica (CWI))
van Vliet, L.J. (TU Delft ImPhys/Quantitative Imaging)
van Leeuwen, Tristan (Universiteit Utrecht)
Kalkman, J. (TU Delft ImPhys/Quantitative Imaging)

2017

As a result of the shallow depth of focus of the optical imaging system, the use of standard filtered back projection in optical projection tomography causes space-variant tangential blurring that increases with the distance to the rotation axis. We present a novel optical tomographic image reconstruction technique that incorporates the point spread function of the imaging lens in an iterative reconstruction. The technique is demonstrated using numerical simulations, tested on experimental optical projection tomography data of single fluorescent beads, and applied to high-resolution emission optical projection tomography imaging of an entire zebrafish larva. Compared to filtered back projection our results show greatly reduced radial and tangential blurring over the entire 5.2x5.2 mm2 field of view, and a significantly improved signal to noise ratio.

Image reconstruction techniques
Inverse problems
omographic image processing

http://resolver.tudelft.nl/uuid:4ed74e00-be0e-4c77-b0b9-74829ce848c7

https://doi.org/10.1088/1361-6560/aa8945

2018-09-20

0031-9155

Physics in Medicine and Biology, 62 (19), 7784-7797

Accepted Author Manuscript

Institutional Repository

journal article

© 2017 A.K. Trull, J. van der Horst, W.J. Palenstijn, L.J. van Vliet, Tristan van Leeuwen, J. Kalkman