Correlating 3D light to 3D electron microscopy for systems biology

Collinson, LM; Carroll, E.C.M.; Hoogenboom, J.P.

doi:10.1016/j.cobme.2017.10.006

Correlating 3D light to 3D electron microscopy for systems biology

Title

Correlating 3D light to 3D electron microscopy for systems biology

Author

Collinson, LM (Francis Crick Institute)
Carroll, E.C.M. (TU Delft ImPhys/Charged Particle Optics)
Hoogenboom, J.P. (TU Delft ImPhys/Charged Particle Optics)

Date

2017-11-15

Abstract

Whilst a ‘resolution revolution’ has taken place at the macromolecular scale in both electron microscopy and light microscopy, a ‘volume revolution’ has taken place at the tissue and organism level in both imaging modalities. At both ends of the scale – resolution and volume – there are concerted efforts to link the information from light and electron microscopes through correlative workflows to link structure to function. Here, we consider the status and potential of correlative imaging in the volume domain (3D CLEM).

Subject

Correlative microscopy
3D CLEM
Large scale imaging
Volume electron microscopy
Ultrastructure
Systems biology

To reference this document use:

http://resolver.tudelft.nl/uuid:4fbe1ab0-a14f-455a-bebe-56a5d37de45c

DOI

https://doi.org/10.1016/j.cobme.2017.10.006

Embargo date

2018-09-01

Source

Current Opinion in Biomedical Engineering, 3, 49-55

Part of collection

Institutional Repository