Adaptive optics in digital micromirror based confocal microscopy

Adaptive optics in digital micromirror based confocal microscopy

Pozzi, P. (TU Delft Team Raf Van de Plas)
Wilding, D. (TU Delft Team Raf Van de Plas)
Soloviev, O.A. (TU Delft Team Raf Van de Plas; Flexible Optical B.V.)
Vdovin, Gleb (TU Delft Team Raf Van de Plas; Flexible Optical B.V.)
Verhaegen, M.H.G. (TU Delft Team Raf Van de Plas)

Bifano, Thomas G. (editor)
Kubby, Joel (editor)
Gigan, Sylvain (editor)

2016

This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through which light propagates. In its most classical form, mostly used in astronomical imaging, adaptive optics is achieved through a closed loop in which the actuators of a deformable mirror are driven by a wavefront sensor. This approach is severely limited in fluorescence microscopy, as the use of a wavefront sensor requires the presence of a bright, point like source in the field of view, a condition rarely satisfied in microscopy samples. Previously reported approaches to adaptive optics in fluorescence microscopy are therefore limited to the inclusion of fluorescent microspheres in the sample, to use as bright stars for wavefront sensors, or time consuming sensorless optimization procedures, requiring several seconds of optimization before the acquisition of a single image.

We propose an alternative approach to the problem, implementing sensorless adaptive optics in a Programmable array microscope. A programmable array microscope is a microscope based on a digital micromirror device, in which the single elements of the micromirror act both as point sources and pinholes.

Confocal Microscopy
Digital Micromirror Device
Wavefront Optimization
Fluorescence

http://resolver.tudelft.nl/uuid:503ef7de-a2a4-4204-ba7b-288a60348b5e

https://doi.org/10.1117/12.2208331

SPIE, Bellingham, WA, USA

978-1-628419511

Proceedings of SPIE: Adaptive Optics and Wavefront Control for Biological Systems II, 9717

Adaptive Optics and Wavefront Control for Biological Systems II, 2016-02-13 → 2016-02-15, San Francisco, United States

Proceedings of SPIE, 1605-7422, 9717

Institutional Repository

conference paper

© 2016 P. Pozzi, D. Wilding, O.A. Soloviev, Gleb Vdovin, M.H.G. Verhaegen