Biocatalytic synthesis of the Green Note trans-2-hexenal in a continuous-flow microreactor

Biocatalytic synthesis of the Green Note trans-2-hexenal in a continuous-flow microreactor

van Schie, M.M.C.H. (TU Delft BT/Biocatalysis)
Pedroso de Almeida, T. (TU Delft BT/Biocatalysis)
Laudadio, Gabriele (Eindhoven University of Technology)
Tieves, F. (TU Delft BT/Biocatalysis)
Fernandez Fueyo, E. (CSIC - Centro de Investigaciones Biologicas (CIB))
Noël, Timothy (Eindhoven University of Technology)
Arends, I.W.C.E. (TU Delft BT/Biotechnologie)
Hollmann, F. (TU Delft BT/Biocatalysis)

BT/Biotechnologie

2018-03-26

The biocatalytic preparation of trans-hex-2-enal from trans-hex-2-enol using a novel aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) is reported. As O₂-dependent enzyme PeAAOx-dependent reactions are generally plagued by the poor solubility of O₂ in aqueous media and mass transfer limitations resulting in poor reaction rates. These limitations were efficiently overcome by conducting the reaction in a flow-reactor setup reaching unpreceded catalytic activities for the enzyme in terms of turnover frequency (up to 38 s^-1) and turnover numbers (more than 300000) pointing towards preparative usefulness of the proposed reaction scheme.

Alcohol oxidase
Alcohol oxidation
Aldehyde
Flow chemistry

http://resolver.tudelft.nl/uuid:6a3f92a7-6a74-4454-bf9c-3f79c180a091

https://doi.org/10.3762/bjoc.14.58

1860-5397

Beilstein Journal of Organic Chemistry, 14, 697-703

Institutional Repository

journal article

© 2018 M.M.C.H. van Schie, T. Pedroso de Almeida, Gabriele Laudadio, F. Tieves, E. Fernandez Fueyo, Timothy Noël, I.W.C.E. Arends, F. Hollmann