Perspectives for the microbial production of methyl propionate integrated with product recovery

Perspectives for the microbial production of methyl propionate integrated with product recovery

Pereira, Joana P.C. (TU Delft BT/Bioprocess Engineering)
van der Wielen, L.A.M. (TU Delft BT/Bioprocess Engineering)
Straathof, Adrie J.J. (TU Delft BT/Bioprocess Engineering)

2018

A new approach was studied for bio-based production of methyl propionate, a precursor of methyl methacrylate. Recombinant E. coli cells were used to perform a cascade reaction in which 2-butanol is reduced to butanone using alcohol dehydrogenase, and butanone is oxidized to methyl propionate and ethyl acetate using a Baeyer-Villiger monooxygenase (BVMO). Product was removed by in situ stripping. The conversion was in line with a model comprising product formation and stripping kinetics. The maximum conversion rates were 1.14 g-butanone/(L h), 0.11 g-ethyl acetate/(L h), and 0.09 g-methyl propionate/(L h). The enzyme regioselectivity towards methyl propionate was 43% of total ester. Starting from biomass-based production of 2-butanol, full-scale ester production with conventional product purification was calculated to be competitive with petrochemical production if the monooxygenase activity and regioselectivity are enhanced, and the costs of bio-based 2-butanol are minimized.

Biomaterials
Bioprocess engineering
Integrated product recovery
Modeling

http://resolver.tudelft.nl/uuid:6c333b21-139c-4569-9e87-a9a40b703b92

https://doi.org/10.1016/j.biortech.2018.01.118

2020-05-01

0960-8524

Bioresource Technology, 256, 187-194

Accepted Author Manuscript

Institutional Repository

journal article

© 2018 Joana P.C. Pereira, L.A.M. van der Wielen, Adrie J.J. Straathof