Print Email Facebook Twitter Diversity of Archaea and detection of crenarchaeotal amoA genes in the rivers Rhine and Têt Title Diversity of Archaea and detection of crenarchaeotal amoA genes in the rivers Rhine and Têt Author Herfort, L. Kim, J.H. Coolen, M.J.L. Abbas, B. Schouten, S. Herndl, G.J. Sinninghe Damste, J.S. Faculty Applied Sciences Department BT/Biotechnology Date 2009-04-28 Abstract Pelagic archaeal phylogenetic diversity and the potential for crenarchaeotal nitrification of Group 1.1a were determined in the rivers Rhine and Têt by 16S rRNA sequencing, catalyzed reported deposition-fluorescence in situ hybridization (CARD–FISH) and quantification of 16S rRNA and functional genes. Euryarchaeota were, for the first time, detected in temperate river water even though a net predominance of crenarchaeotal phylotypes was found. Differences in phylogenic distribution were observed between rivers and seasons. Our data suggest that a few archaeal phylotypes (Euryarchaeota Groups RC-V and LDS, Crenarchaeota Group 1.1a) are widely distributed in pelagic riverine environments whilst others (Euryarchaeota Cluster Sagma-1) may only occur seasonally in river water. Crenarchaeota Group 1.1a has recently been identified as a major nitrifier in the marine environment and phylotypes of this group were also present in both rivers, where they represented 0.3% of the total pelagic microbial community. Interestingly, a generally higher abundance of Crenarchaeota Group 1.1a was found in the Rhine than in the Têt, and crenarchaeotal ammonia monooxygenase gene (amoA) was also detected in the Rhine, with higher amoA copy numbers measured in February than in September. This suggests that some of the Crenarchaeota present in river waters have the ability to oxidize ammonia and that riverine crenarchaeotal nitrification of Group 1.1a may vary seasonally. Subject archaeariverdiversitynitrification To reference this document use: http://resolver.tudelft.nl/uuid:6d09f891-d1be-4ffb-90d3-f7eb1be4a0b5 Publisher Inter-Research ISSN 0948-3055 Source https://doi.org/10.3354/ame01294 Source Aquatic Microbial Ecology, 55 (2), 2009 Part of collection Institutional Repository Document type journal article Rights © 2009 Inter-Research Files PDF Abbas_2009.pdf 1.34 MB Close viewer /islandora/object/uuid:6d09f891-d1be-4ffb-90d3-f7eb1be4a0b5/datastream/OBJ/view