Print Email Facebook Twitter ATP measurement in seawater reverse osmosis systems Title ATP measurement in seawater reverse osmosis systems: Eliminating seawater matrix effects using a filtration-based method Author Abushaban, Almotasembellah (IHE Delft Institute for Water Education) Salinas Rodriguez, S.G. (IHE Delft Institute for Water Education) Mangal, M.N. (TU Delft Sanitary Engineering; IHE Delft Institute for Water Education) Mondal, Subhanjan (Promega Corporation) Goueli, Said A. (Promega Corporation; University of Wisconsin School of Medicine and Public Health) Knezev, Aleksandra (Het Waterlaboratorium) Vrouwenvelder, J.S. (TU Delft BT/Environmental Biotechnology; King Abdullah University of Science and Technology) Schippers, Jan C. (IHE Delft Institute for Water Education) Kennedy, M.D. (TU Delft Sanitary Engineering; IHE Delft Institute for Water Education) Date 2019-03-01 Abstract A direct method for measuring adenosine-triphosphate (ATP) in seawater was developed recently, in which commercial reagents are added directly to seawater. However, calibration is required if seawater quality changes (such as changes in salinity, pH, Mg2+, Fe3+) as the seawater matrix interferes with ATP measurement. In this research, a 0.1 μm filtration process is introduced to eliminate such interferences. In addition, a filter rinsing step with sterilized artificial seawater is proposed to eliminate interference of free ATP. The ATP-filtration method is fast (<5 min), reproducible (VC = 7%), six times more sensitive than the direct ATP-method and correlates (R2 = 0.72, n = 100) with intact cell concentration. Microbial ATP concentration measured using the ATP-filtration method and the ATP-direct method were comparable. Microbial ATP measured along the treatment train of a full-scale seawater reverse osmosis (SWRO) plant decreased from 530 in the raw seawater to 10 ng-ATP/L after pre-treatment and to 0.5 ng-ATP/L in the SWRO permeate. The method was also applied to monitor bacterial growth potential (BGP) across the pre-treatment train of a (pilot) seawater desalination plant, where the removal of BGP through the media filtration and ultrafiltration was 44% and 7%, respectively. Subject Adenosine-triphosphateBiofoulingFiltration based ATP methodPre-treatmentRO permeateSeawater reverse osmosis To reference this document use: http://resolver.tudelft.nl/uuid:79a0ab44-4922-499e-97da-b4b23587194a DOI https://doi.org/10.1016/j.desal.2018.11.020 ISSN 0011-9164 Source Desalination, 453, 1-9 Part of collection Institutional Repository Document type journal article Rights © 2019 Almotasembellah Abushaban, S.G. Salinas Rodriguez, M.N. Mangal, Subhanjan Mondal, Said A. Goueli, Aleksandra Knezev, J.S. Vrouwenvelder, Jan C. Schippers, M.D. Kennedy Files PDF 1_s2.0_S0011916418319064_main.pdf 833.48 KB Close viewer /islandora/object/uuid:79a0ab44-4922-499e-97da-b4b23587194a/datastream/OBJ/view