Print Email Facebook Twitter A Mismatch Relaxation to the Primer Selection Process of an Amplicon Sequencing Algorithm Title A Mismatch Relaxation to the Primer Selection Process of an Amplicon Sequencing Algorithm Author Polimac, Dean (TU Delft Electrical Engineering, Mathematics and Computer Science) Contributor Baaijens, J.A. (mentor) van Bemmelen, J. (mentor) Raman, C.A. (graduation committee) Degree granting institution Delft University of Technology Programme Computer Science and Engineering Project CSE3000 Research Project Date 2024-02-02 Abstract In this study we introduce a different approach to the primer selection problem in the AmpliDiff [12] algorithm. Two different metrics, being the Hamming distance [2], and the Levenshtein distance [4], are used to compute sets of similar primers. This is done such that locations where mismatches between the primers and the target sequence can occur are determined by the locations of similar primers. The impact that said mismatches have on the solution set of amplicons and their respective primers is outlined. With this we show potential benefits of allowing mismatches to occur, as well as their drawbacks. Subject Primer SelectionPrimer MismatchAmpliDiff To reference this document use: http://resolver.tudelft.nl/uuid:7c4e99dc-3af0-4187-8988-f8862924414d Part of collection Student theses Document type bachelor thesis Rights © 2024 Dean Polimac Files PDF CSE3000_Final_Paper.pdf 346.25 KB Close viewer /islandora/object/uuid:7c4e99dc-3af0-4187-8988-f8862924414d/datastream/OBJ/view