Halanaeroarchaeum sulfurireducens gen. nov., sp. nov., the first obligately anaerobic sulfur-respiring haloarchaeon, isolated from a hypersaline lake

Halanaeroarchaeum sulfurireducens gen. nov., sp. nov., the first obligately anaerobic sulfur-respiring haloarchaeon, isolated from a hypersaline lake

Sorokin, Dimitry Y. (TU Delft BT/Environmental Biotechnology; Russian Academy of Sciences)
Kublanov, Ilya V. (Russian Academy of Sciences)
Yakimov, Mikhail M. (IAMC-CNR)
Rijpstra, W. Irene C (Universiteit Utrecht)
Sinninghe Damsté, Jaap S. (Universiteit Utrecht)

2016-06-01

Anaerobic enrichments with acetate as electron donor and carbon source, and elemental sulfur as electron acceptor at 4 M NaCl using anaerobic sediments and brines from several hypersaline lakes in Kulunda Steppe (Altai, Russia) resulted in isolation in pure culture of four strains of obligately anaerobic haloarchae growing exclusively by sulfur respiration. Such metabolism has not yet been demonstrated in any known species of Halobacteria, and in the whole archaeal kingdom, acetate oxidation with sulfur as acceptor was not previously demonstrated. The four isolates had nearly identical 16S rRNA gene sequences and formed a novel genus-level branch within the family Halobacteriaceae. The strains had a restricted substrate range limited to acetate and pyruvate as electron donors and elemental sulfur as electron acceptor. In contrast to aerobic haloarchaea, the biomass of anaerobic isolates completely lacked the typical red pigments. Growth with acetate+sulfur was observed between 3–5 M NaCl and at a pH range from 6.7 to 8.0. The membrane core lipids were dominated by archaeols. On the basis of distinct physiological and phylogenetic data, the sulfur-respiring isolates represent a novel species of a new genus in the family Halobacteriaceae, for which the name Halanaeroarchaeaum sulfurireducens gen. nov., sp. nov. is proposed. The type strain of the type species is HSR2^T (=JCM 30661^T=UNIQEM U935^T).

Anaerobic
Haloarchaea
Hypersaline lakes
Sulfur reduction

http://resolver.tudelft.nl/uuid:81dfdca8-a977-40ae-be1b-a1ae92d6a2e5

https://doi.org/10.1099/ijsem.0.001041

2017-06-10

1466-5026

International Journal of Systematic and Evolutionary Microbiology, 66 (6), 2377-2381

Accepted Author Manuscript

Institutional Repository

journal article

© 2016 Dimitry Y. Sorokin, Ilya V. Kublanov, Mikhail M. Yakimov, W. Irene C Rijpstra, Jaap S. Sinninghe Damsté