The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus

The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus

Pelkmans, Jordi F. (Universiteit Utrecht)
Vos, A.M. (Universiteit Utrecht)
Scholtmeijer, Karin (Universiteit Utrecht)
Hendrix, Ed (Wageningen University & Research)
Baars, Johan J.P. (Wageningen University & Research)
Gehrmann, T. (TU Delft Pattern Recognition and Bioinformatics)
Reinders, M.J.T. (TU Delft Pattern Recognition and Bioinformatics)
Lugones, Luis G. (Universiteit Utrecht)
Wösten, Han A.B. (Universiteit Utrecht)

2016

The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

Fungi
Basidiomycete
Agaricus bisporus
Mushroom
Transcription factor
Cys2His2

http://resolver.tudelft.nl/uuid:87528d08-1afc-4184-bfb9-f100a5d2e404

https://doi.org/10.1007/s00253-016-7574-9

0175-7598

Applied Microbiology and Biotechnology, 100 (16), 7151-7159

Institutional Repository

journal article

© 2016 Jordi F. Pelkmans, A.M. Vos, Karin Scholtmeijer, Ed Hendrix, Johan J.P. Baars, T. Gehrmann, M.J.T. Reinders, Luis G. Lugones, Han A.B. Wösten