Print Email Facebook Twitter Combination of bone morphogenetic protein-2 plasmid DNA with chemokine CXCL12 creates an additive effect on bone formation onset and volume Title Combination of bone morphogenetic protein-2 plasmid DNA with chemokine CXCL12 creates an additive effect on bone formation onset and volume Author Wegman, F.C.M. (TU Delft Transport and Planning; University Medical Center Utrecht) Poldervaart, M. T. (University Medical Center Utrecht) van der Helm, Y. J. (University Medical Center Utrecht) Oner, F. C. (University Medical Center Utrecht) Dhert, W. J. (Universiteit Utrecht) Alblas, J. (University Medical Center Utrecht) Date 2015-07-27 Abstract Bone morphogenetic protein-2 (BMP-2) gene delivery has shown to induce bone formation in vivo in cell-based tissue engineering. In addition, the chemoattractant stromal cell-derived factor-1α (SDF-1α, also known as CXCL12) is known to recruit multipotent stromal cells towards its release site where it enhances vascularisation and possibly contributes to osteogenic differentiation. To investigate potential cooperative behaviour for bone formation, we investigated combined release of BMP-2 and SDF-1α on ectopic bone formation in mice. Multipotent stromal cellseeded and cell-free constructs with BMP-2 plasmid DNA and /or SDF-1α loaded onto gelatin microparticles, were implanted subcutaneously in mice for a period of 6 weeks. Histological analysis and histomorphometry revealed that the onset of bone formation and the formed bone volume were both enhanced by the combination of BMP-2 and SDF-1α compared to controls in cell-seeded constructs. Samples without seeded multipotent stromal cells failed to induce any bone formation. We conclude that the addition of stromal cellderived factor-1α to a cell-seeded alginate based bone morphogenetic protein-2 plasmid DNA construct has an additive effect on bone formation and can be considered a promising combination for bone regeneration. Subject Animal modelBoneBone morphogenetic protein-2Controlled releaseGene therapyMultipotent stromal cellsStromal cell-derived factor-1αTissue engineering To reference this document use: http://resolver.tudelft.nl/uuid:94f68c74-67c5-454e-87b6-a1f42cddf3d0 ISSN 1473-2262 Source European Cells & Materials, 30, 1-11 Part of collection Institutional Repository Document type journal article Rights © 2015 F.C.M. Wegman, M. T. Poldervaart, Y. J. van der Helm, F. C. Oner, W. J. Dhert, J. Alblas Files PDF v030a01.pdf 1.6 MB Close viewer /islandora/object/uuid:94f68c74-67c5-454e-87b6-a1f42cddf3d0/datastream/OBJ/view