Print Email Facebook Twitter Production and immobilization of lipase PCL and its application in synthesis of α‐linolenic acid‐rich diacylglycerol Title Production and immobilization of lipase PCL and its application in synthesis of α‐linolenic acid‐rich diacylglycerol Author Liu, Nan (South China University of Technology) Liu, D. (South China University of Technology) Wang, Weifei (Guangdong Academy of Agricultural Sciences) Hollmann, F. (TU Delft BT/Biocatalysis) Xu, Long (South China University of Technology) Ma, Yunjian (South China University of Technology) Yang, Bo (South China University of Technology) Bai, Weidong (Zhongkai University of Agriculture and Engineering) Sun, Xiaotao (Beijing Technology and Business University) Wang, Yonghua (South China University of Technology) Date 2018 Abstract α‐Linolenic acid‐rich diacylglycerol has been demonstrated with promising health promotion functions. This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of α‐linolenic acid with glycerol. The resin ECR8806 was selected as an effective support for the immobilization of lipase PCL. Fourier transform infrared and Laser scanning confocal microscope analysis proved that the lipase was successfully immobilized on the resin. Compared with the free PCL, the immobilized one exhibited higher temperature tolerance. Under optimized reaction conditions, a DAG content of 54.49% were obtained. After further purified by molecular distillation, the purity of DAG was up to 99.28%. During esterification, the immobilized PCL was quite stable and retained more than 91.60% of its initial activity after 10 cycles. These new findings on the immobilized PCL will make it to be a prospective enzyme in oils and fats industry. Subject diacylglycerolesterificationimmobilizationreusabilitya-Linolenic acid To reference this document use: http://resolver.tudelft.nl/uuid:9848d77d-a98a-45c5-8077-aa3735d53f04 DOI https://doi.org/10.1111/jfbc.12574 Embargo date 2019-01-23 ISSN 1745-4514 Source Journal of Food Biochemistry Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2018 Nan Liu, D. Liu, Weifei Wang, F. Hollmann, Long Xu, Yunjian Ma, Bo Yang, Weidong Bai, Xiaotao Sun, Yonghua Wang Files PDF Liu_et_al_2018_Journal_of ... mistry.pdf 846.44 KB Close viewer /islandora/object/uuid:9848d77d-a98a-45c5-8077-aa3735d53f04/datastream/OBJ/view