Print Email Facebook Twitter The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways Title The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways Author Sousa, Diana Z. (Wageningen University & Research) Visser, M. (Wageningen University & Research) Van Gelder, Antonie H. (Wageningen University & Research) Boeren, Sjef (Wageningen University & Research) Pieterse, M.M. (TU Delft OLD BT/Analytical Biotechnology; Netherlands Proteomics Centre) Pinkse, M.W.H. (TU Delft OLD BT/Analytical Biotechnology; Netherlands Proteomics Centre) Verhaert, P.D.E.M. (TU Delft BT/Afdelingsbureau; Netherlands Proteomics Centre; Universiteit Maastricht; Janssen Pharmaceutica Campus) Vogt, Carsten (Helmholtz Centre for Environmental Research - UFZ) Franke, Steffi (Helmholtz Centre for Environmental Research - UFZ) Kümmel, Steffen (Helmholtz Centre for Environmental Research - UFZ) Stams, Alfons J.M. (Wageningen University & Research; University of Minho) Date 2018-01-16 Abstract Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment. To reference this document use: http://resolver.tudelft.nl/uuid:9e98b8e1-f2dd-47b9-9b67-96e3ea34f033 DOI https://doi.org/10.1038/s41467-017-02518-9 ISSN 2041-1723 Source Nature Communications, 9 (1) Part of collection Institutional Repository Document type journal article Rights © 2018 Diana Z. Sousa, M. Visser, Antonie H. Van Gelder, Sjef Boeren, M.M. Pieterse, M.W.H. Pinkse, P.D.E.M. Verhaert, Carsten Vogt, Steffi Franke, Steffen Kümmel, Alfons J.M. Stams Files PDF s41467_018_03902_9.pdf 1.16 MB Close viewer /islandora/object/uuid:9e98b8e1-f2dd-47b9-9b67-96e3ea34f033/datastream/OBJ/view