Towards preparative peroxygenase-catalyzed oxyfunctionalization reactions in organic media

Title

Towards preparative peroxygenase-catalyzed oxyfunctionalization reactions in organic media

 Author

Fernandez Fueyo, E. (TU Delft BT/Biocatalysis)
Ni, Y. (TU Delft BT/Biocatalysis)
Gomez Baraibar, A. (TU Delft BT/Biocatalysis)
Alcalde, Miguel (University of the Balearic Islands)
van Langen, L.M. (ViaZym)
Hollmann, F. (TU Delft BT/Biocatalysis) ORCID 0000-0003-4821-756X

 Date

2016

 Abstract

The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions. The stereoselective hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as reaction medium together with the immobilized biocatalyst and tertBuOOH as oxidant. Stability and activity issues still have to be addressed. Nevertheless, gram-scale production of enantiopure (R)-1-phenylethanol was achieved with respectable 90,000 turnovers of the biocatalyst.


 Subject

Biocatalysis
Hydroxylation
Non-aqueous reaction media
Oxyfunctionalization
Peroxygenase

 To reference this document use:

http://resolver.tudelft.nl/uuid:b79f43a7-febf-4982-b68f-decc63acc5d2

 DOI

https://doi.org/10.1016/j.molcatb.2016.09.013

 ISSN

1381-1177

 Source

Journal of Molecular Catalysis B: Enzymatic, 134, 347-352

 Part of collection

Institutional Repository

 Document type

journal article

 Rights

© 2016 E. Fernandez Fueyo, Y. Ni, A. Gomez Baraibar, Miguel Alcalde, L.M. van Langen, F. Hollmann
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