Print Email Facebook Twitter Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS) Title Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS) Author Felz, S. (TU Delft BT/Environmental Biotechnology; TU Delft OLD BT/Cell Systems Engineering) Vermeulen, Pascalle (Student TU Delft) van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology; TU Delft OLD BT/Cell Systems Engineering) Lin, Yue Mei (Student TU Delft) Date 2019 Abstract Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on their applicability for EPS analysis. EPS fractions of interest were proteins, sugars, uronic acids and phenolic compounds. The applied methods (Lowry method, bicinchoninic acid assay, phenol sulfuric acid method, carbazole sulfuric acid method) were investigated in terms of their sensitivity towards the selected standard compound. Interference of compounds present in EPS with the colorimetric methods was further evaluated. All methods showed to be highly depending on the choice of standard compound and susceptible towards interference by compounds present in EPS. This study shows that currently used colorimetric methods are not capable of accurately characterizing EPS. More advanced methods are needed to be able to draw conclusions about biofilm composition, structure and functionality. Subject Aerobic granular sludgeBiofilmColorimetric methodsEPS analysisExtracellular polymeric substances (EPS) To reference this document use: http://resolver.tudelft.nl/uuid:b7b7b4fb-e4ee-474e-a02f-fe214ca68973 DOI https://doi.org/10.1016/j.watres.2019.03.068 ISSN 0043-1354 Source Water Research, 157, 201-208 Part of collection Institutional Repository Document type journal article Rights © 2019 S. Felz, Pascalle Vermeulen, Mark C.M. van Loosdrecht, Yue Mei Lin Files PDF 1_s2.0_S004313541930209X_main.pdf 842.08 KB Close viewer /islandora/object/uuid:b7b7b4fb-e4ee-474e-a02f-fe214ca68973/datastream/OBJ/view